GrainGenes Sequence Report: BE705465
Sequence
BE705465
External Databases
Data at GenBank Data at EMBL Data at DDBJ
DB Remark
Locus Source: Secale cereale (rye)
Keyword
EST
Species
Secale cereale
Cultivar
Puma (winter rye)
Clone Library
Sc01_AAFC_ECORC_cold_stressed_winter_rye_seedl ings
Tissue
leaf, crown
Developmental Stage
seedling three-leaf stage
Data Source
genbank Release 135, Apr 15 2003 genbank Downloaded 2008-2009
Title
Sc01_01b01_R Sc01_AAFC_ECORC_cold_stressed_winter_rye_seedlings Secale cereale cDNA clone Sc01_01b01, mRNA sequence.
Clone
Sc01_01b01
Probe
SCM157
Remark
DB_xref: taxon:4550 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Singh,J.A.; Eastern Cereal and Oilseed Research Centre; Agriculture and Agri-food Canada; KW Neatby Bldg., Central Experimental Farm, Ottawa, Ontario, K1A; 0C6, Canada; Tel: (613) 759-1662; Fax: (613) 759-1701; Email: singhja@em.agr.ca. Locus Comment: Contact: Singh,J.A.; Eastern Cereal and Oilseed Research Centre; Agriculture and Agri-food Canada; KW Neatby Bldg., Central Experimental Farm, Ottawa, Ontario, K1A; 0C6, Canada; Tel: (613) 759-1662; Fax: (613) 759-1701; Email: singhja@agr.gc.ca. Note: Vector: Bluescript SK-/XhoI-EcoRI; Site_1: Eco RI; Site_2: Xho I; Sampled three-leaf seedlings treated for one week at 2oC, 12 hrs light/day. Library made with Stratagene UNIZAP XR Kit/Gigapack III Gold Kit. Lambda library is amplified, then mass excised in SOLR cells.
DNA
ccacgtcgacgacaagccgggctacaccacggaggagcgcgtgckgcgcg
ccgttgctgtaagccgggaacgcttgtcctacatgcagcagcagcagcag
cgtcgggcgttcggcgacgtgagcdcgcctgtccacctggccacccggca
gtacatcgccgagtacctcatcggcgacccgccccagcgcgcggacgccc
tcatcgacaccggcagcaacctcatctggacccagtgckcgacgacgtgc
ctcaaggcgtgcgccaagcaggacntgccctactacaacctgtcgagctc
cgcgagcttcgcgcccgtgccgtgcaccgacagcgccaagctgtgcgcgg
ccaacggcgtgcacctctgcggcctggaccgcagctgcaccttcgtcgcc
agctacggcgccgggagcatcatcgggtcgctcggcaccgaggccttcac
cttccagtccggcgcggcgaggctcgccttcgggtgcgtgagcctgacgc
ggatcaccaagggcgccctgaacggcgcgtccgggctcatcgggctcggc
cgcggccgcctgtcactcgtctcccagacgggcgccacaaagttctccta
ctgcctcgccccctacttccacaaccacggcgcctccagccacctgttcg
tcggcgcgtcggcgagcctgagcagcggcggcgcgtgacatccatgccgt
tcgtgagagcccaaggat

GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Sequence Report: BE705465
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