GrainGenes Sequence Report: BE705259
Sequence
BE705259
External Databases
Data at GenBank Data at EMBL Data at DDBJ
DB Remark
Locus Source: Secale cereale (rye)
Keyword
EST
Species
Secale cereale
Cultivar
Puma (winter rye)
Clone Library
Sc02_AAFC_ECORC_cold_stressed_winter_rye_seedl ings
Tissue
leaf, crown
Developmental Stage
seedling three-leaf stage
Data Source
genbank Release 135, Apr 15 2003 genbank Downloaded 2008-2009
Title
Sc02_10c05_A Sc02_AAFC_ECORC_cold_stressed_winter_rye_seedlings Secale cereale cDNA clone Sc02_10c05, mRNA sequence.
Clone
Sc02_10c05
Probe
SCM155
Remark
DB_xref: taxon:4550 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Singh,J.A.; Eastern Cereal and Oilseed Research Centre; Agriculture and Agri-food Canada; KW Neatby Bldg., Central Experimental Farm, Ottawa, Ontario, K1A; 0C6, Canada; Tel: (613) 759-1662; Fax: (613) 759-1701; Email: singhja@em.agr.ca. Locus Comment: Contact: Singh,J.A.; Eastern Cereal and Oilseed Research Centre; Agriculture and Agri-food Canada; KW Neatby Bldg., Central Experimental Farm, Ottawa, Ontario, K1A; 0C6, Canada; Tel: (613) 759-1662; Fax: (613) 759-1701; Email: singhja@agr.gc.ca. Note: Vector: Bluescript SK+/XhoI-EcoRI; Site_1: Eco RI; Site_2: Xho I; Sampled three-leaf seedlings treated for one week at 2oC, 12 hrs light/day. Library made with Stratagene UNIZAP XR Kit/(not packaged). cDNA is directly ligated into SK+/XhoI-EcoRI, then electroporated into TOP10 cells (Invitrogen).
DNA
caacgtggaataaatacctcaccgacagtnaaacgcaaacaaaacacaat
ttagtagtgataaccaacactaaattctcctttcctttttacacctatac
ccctcgacgctaacacttattaccgccatgtgccacacgccttggccttc
agccttgatgatcttcttcttcgctacgcacacccgcgcgcgccgcccgc
cgccgacggagcagaggagggggaggaggaggaagaagaagaagaagcag
agcgcgtacggacggctagacctcgacgtggcagtaggtggcggcctgga
acctgagcccgctggcccccggcgcgccgttgccgccggcgccgacgagg
aaggcggggcaggtggcgaagatgtggtagtggccggagatccagctgcc
caccttccaccggacgcggccgtcgatcttgacctggaggatgaggtacc
cggcctggacgtccttggcgagcgcgtcggcgaggtagggcgcgaagggg
acgctggggccggacagcaccggggaccagacctccacgtcgccgtggcc
ctggtacaccggcgggagcgcggcggccagcgtgatctgctggtacttgt
aggaggcgtarmgtcgaggcggtcgtagtagacgcgacgcggtcgttggg
gttgcgggaggcgacggtgacctggagcacggtggagacacgccggcggs
gscggaggcgttggcggtgagcacgtccagctgccggagcgaggcgtcct
ggaggtagaagcgcgggtgcgtggggcggaggacgaggtaracgatgagc
gcgatgacggcaccaccagggccaggaaga
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Sequence Report: BE705259
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||