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GrainGenes Sequence Report: BE704638

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Sequence
BE704638
External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
TIGR Gene Index
TC4003
DB Remark
Locus Source: Secale cereale (rye)
Keyword
EST
Species
Secale cereale
Cultivar
Puma (winter rye)
Clone Library
Sc01_AAFC_ECORC_cold_stressed_winter_rye_seedl ings
Tissue
leaf, crown
Developmental Stage
seedling three-leaf stage
Data Source
genbankRelease 135, Apr 15 2003
genbankDownloaded 2008-2009
Title
Sc01_04g07_A Sc01_AAFC_ECORC_cold_stressed_winter_rye_seedlings Secale cereale cDNA clone Sc01_04g07, mRNA sequence.
Clone
Sc01_04g07
Probe
SCM183
REMS1253
Remark
DB_xref: taxon:4550
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Singh,J.A.; Eastern Cereal and Oilseed Research Centre; Agriculture and Agri-food Canada; KW Neatby Bldg., Central Experimental Farm, Ottawa, Ontario, K1A; 0C6, Canada; Tel: (613) 759-1662; Fax: (613) 759-1701; Email: singhja@em.agr.ca.
Locus Comment: Contact: Singh,J.A.; Eastern Cereal and Oilseed Research Centre; Agriculture and Agri-food Canada; KW Neatby Bldg., Central Experimental Farm, Ottawa, Ontario, K1A; 0C6, Canada; Tel: (613) 759-1662; Fax: (613) 759-1701; Email: singhja@agr.gc.ca.
Note: Vector: Bluescript SK-/XhoI-EcoRI; Site_1: Eco RI; Site_2: Xho I; Sampled three-leaf seedlings treated for one week at 2oC, 12 hrs light/day. Library made with Stratagene UNIZAP XR Kit/Gigapack III Gold Kit. Lambda library is amplified, then mass excised in SOLR cells.
DNA
atgacattgcttcatcggtgcgcatcagggtangcggatcaatcccacat
atgtccagacgatcaactagaaatatagatagatagatagatagatacag
ggttggtagatgggatagcattggtgaagctccttctcatcagcttctca
caccaaaagagcaccgtcatgggattaggcccccaccaagaaaggccagc
gagcgttgttggcctgaacgtgccgctcatagatgtcgtcgtcctgcgag
tcgtagccgtccgccacatccccatccgagtattcctcttggtccgtggg
tggcttcgtggcctcgatccacgccaggaacgcagccccctcctcgtccc
gctccaattccggcttctcttggagccgcacttgtccgcctccgcctccg
cctccgccttggcgtcgaggaaatcctcctccttctccagcagcgccacg
aacttgaggagcgcctcctcctcctccgtggtcatggtcttcaactccac
cttgcccttggcctcggcctccttcaccttggccttgacctccttcacct
tggccttgacctccggcttggacttggcctccttcaccttggccttggcc
ttgaccgccggcttcaccttggccttctccaccttgagcttggccttctt
caccttggccttgaggatgggcgcctcctgctccgccctcaacttaacgg
tgaaggcctcctggaacgaggtgaaggggaaattctccaagccggcgggc
aacggcatggacaagtgcatcttctcgacggccatcgccggatctatcct
ctcctctcctgcttgcttaaattagggttag

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