GrainGenes Sequence Report: BE704499
Sequence
BE704499
External Databases
Data at GenBank Data at EMBL Data at DDBJ
DB Remark
Locus Source: Secale cereale (rye)
Keyword
EST
Species
Secale cereale
Cultivar
Puma (winter rye)
Clone Library
Sc01_AAFC_ECORC_cold_stressed_winter_rye_seedl ings
Tissue
leaf, crown
Developmental Stage
seedling three-leaf stage
Data Source
genbank Release 135, Apr 15 2003 genbank Downloaded 2008-2009
Title
Sc01_05f07_A Sc01_AAFC_ECORC_cold_stressed_winter_rye_seedlings Secale cereale cDNA clone Sc01_05f07, mRNA sequence.
Clone
Sc01_05f07
Probe
SCM176 REMS1247
Remark
DB_xref: taxon:4550 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Singh,J.A.; Eastern Cereal and Oilseed Research Centre; Agriculture and Agri-food Canada; KW Neatby Bldg., Central Experimental Farm, Ottawa, Ontario, K1A; 0C6, Canada; Tel: (613) 759-1662; Fax: (613) 759-1701; Email: singhja@em.agr.ca. Locus Comment: Contact: Singh,J.A.; Eastern Cereal and Oilseed Research Centre; Agriculture and Agri-food Canada; KW Neatby Bldg., Central Experimental Farm, Ottawa, Ontario, K1A; 0C6, Canada; Tel: (613) 759-1662; Fax: (613) 759-1701; Email: singhja@agr.gc.ca. Note: Vector: Bluescript SK-/XhoI-EcoRI; Site_1: Eco RI; Site_2: Xho I; Sampled three-leaf seedlings treated for one week at 2oC, 12 hrs light/day. Library made with Stratagene UNIZAP XR Kit/Gigapack III Gold Kit. Lambda library is amplified, then mass excised in SOLR cells.
DNA
cgaggcaaaaacataaggvatccagtccaccaacatttacatggcacccc
acgaacccaaaagaatggaaagaaccaccagcagcacacactcggtatct
atacgtagcaacatcagatctactacccacaaaaataaacagcacggtaa
actacagctctaaatccccagatatactgtaattgcgttgcatcttcacc
atcgatctcacctgagacctgaatctgaatattcttcttcttcttcttct
tcccgtcgtcgtatctatttggrtctcgcggacgccgccaccagatccta
gccttgctcccagccgaagaacaagatgcgtggcaaactgcttaatgcgc
cattgcktggccgtactggaagctctgggtgtaggcgaactgcggcgcgg
gcktgcagtggtggtgcgcggaggacggggcaggcagcgcgcccttgcgg
ccctgcgtggtgggcagcgagtggtcggagacgacgaggcggcggacccc
ggagcgcacttgttagaggacgtgggcccaggtgcccctgctgttgcggc
cgagcgcggccgcctcctcctcgttcatctcgaggtcgccggcgaasagg
ctttctttggagagcagccagccgaggccccagtcccaccagcgcttgag
cgggccgccggcgaactcggcggcgagcgagggcgccgacctggtgcggc
ggaacccacatcggcggacgcggaggagatgggctggatcttgccggaga
agctggagaggrcgctksscaggcccatgaggtggstctcctccttgctc
c

GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Sequence Report: BE704499
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