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GrainGenes Sequence Report: BE637891

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Sequence
BE637891
Contig
NSFT03P2_Contig2066
Tracefile
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External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
TIGR Gene Index
TC279970
wEST map position
BE637891
NCBI UniGene
Ta.11481
DB Remark
Locus Source: Triticum aestivum (bread wheat)
UniGene title 'Transcribed locus, weakly similar to NP_572749.2 CG1578 [Drosophila melanogaster]'
Keyword
EST
Germplasm
Chinese Spring
Species
Triticum aestivum
Cultivar
Chinese Spring
Chromosome
6AL
6BL
6DL
Clone Library
Wheat pre-anthesis spike cDNA library
Tissue
Spike before anthesis
Developmental Stage
Adult plant
Data Source
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
WHE1755-1758_E05_E05ZS Wheat pre-anthesis spike cDNA library Triticum aestivum cDNA clone WHE1755-1758_E05_E05, mRNA sequence.
Other Name
WHE1755-1758_E05_E05ZS  [ wEST-mySQL (obsolete) ]
Strain
lab_host E. coli SOLR
DNA Library
TA019E1X
Clone
WHE1755-1758_E05_E05
Probe
WHE1755-1758_E05_E05
Remark
DB_xref: taxon:4565
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: Stratagene SK primer.
Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown in the greenhouse. Whole spike with awns trimmed, white, green and yellow anther were collected and total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA Homology
TaAffx.122098.1.S1_at60WHE2AFFY
DNA
atcggctcaaggacggagggttaagttgggcaaatgttgtatatgttcac
ctctacatttcaagcatgaaggaatttggattggccaatgaagtttatgt
cagcttcatcacagaaaagaaatgtcaccttggtgttccttcgcgcagta
caattgaattaccacttgttcaggctggtttgggccatgcatatgtggag
gttctagttgcaagtgatctggtcaaaagagttctgcacgtacaaagcat
ctcatgctgggctcctagttgcattggaccttatagccaggcaacacttt
atggagagattctctatatggctggtcagctcgggcttgacccccctaca
atgaagctctgtcctggaggcgcaacagctcaactggaactagcactgcg
aaatagtgaagctgtggctaatgccttcaagagctctatcttctcttcag
cagtacactttctggtgtactgctctgcccacctgacatccactgagaag
gaagcagttgaacaaaagctgcagaattcatatattgctcatttggattc
cgcaagaagtggatcatatcctactattctatatgtactagcaccggatc
ttccaaaaggagcatgtgtggagataaaaccaacgttgtatgtccccact
gatgac

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