GrainGenes Sequence Report: NSFT03P2

UniGene title 'Transcribed locus, strongly similar to NP_196598.1 EMB3010 (EMBRYO DEFECTIVE 3010); structural constituent of ribosome [Arabidopsis thaliana]'

Keyword

EST

Germplasm

Chinese Spring

Species

Triticum aestivum

Cultivar

Chinese Spring

Clone Library

Wheat pre-anthesis spike cDNA library

Tissue

Spike before anthesis

Developmental Stage

Adult plant

Data Source

genbank	Release 135, Apr 15 2003
genbank	Updated Nov 2006

Title

WHE0983-0986_G11_G11ZS Wheat pre-anthesis spike cDNA library Triticum aestivum cDNA clone WHE0983-0986_G11_G11, mRNA sequence.

Other Name

WHE0983-0986_G11_G11ZS [ wEST-mySQL (obsolete) ]

Strain

lab_host E. coli SOLR

DNA Library

TA019E1X

Clone

WHE0983-0986_G11_G11

Probe

[Wcl125ct578cn920]

{SpCl-194}

BE637639

Remark

DB_xref: taxon:4565

Feature: source: mol_type = 'mRNA';

Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: Stratagene SK primer.

Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown in the greenhouse. Whole spike with awns trimmed, white, green and yellow anther were collected and total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).

DNA

aaccgcgccgccgccgccgccgctccacccagcgttcggtttgctcgccg
ccggcgagctcgtgccgcgactccaccatgaagctgaacatcgcgaaccc
caccacggggtgccagaagaaggtcgagatcgatgacgaccagaagctgc
ggaacctttatgacaagagaatctcccaggaggtagttggtgatcttctg
ggtgaggaattcaagggggatatcttcaagatcatgggtggctgcgataa
gcagggtttcccaatgaagcaaggagtgctaacttctgggcgtgtccgcc
ttctgcttcacaggggcgcaccttgcttccgtgggtttggcaggcgtaat
ggtgagcgcaggaggaagtccgtccgtggttgcattgtcagccaagacct
gtctgttatcaacttggtgattgtc


GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.