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GrainGenes Sequence Report: Ta.27713.2.A1_x_at

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Sequence
BE637463
Contig
Ta.27713.2.A1_x_at
NSFT03P2_Contig18494
External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
TIGR Gene Index
TC333449
wEST map position
BE637463
NCBI UniGene
Ta.54858
DB Remark
Locus Source: Triticum aestivum (bread wheat)
UniGene title 'Elongation factor-1 alpha'
Keyword
EST
Germplasm
Chinese Spring
Species
Triticum aestivum
Cultivar
Chinese Spring
Chromosome
5BL
5DL
Clone Library
Wheat 20-45 DAP spike cDNA library
Tissue
Spike and seed
Developmental Stage
Adult plant
Data Source
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
WHE0859_F01_K01ZS Wheat 20-45 DAP spike cDNA library Triticum aestivum cDNA clone WHE0859_F01_K01, mRNA sequence.
Other Name
WHE0859_F01_K01ZS  [ wEST-mySQL (obsolete) ]
Strain
lab_host E. coli SOLR
DNA Library
TA017E1X
Clone
WHE0859_F01_K01
Probe
WHE0859_F01_K01
Remark
DB_xref: taxon:4565
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: Stratagene SK primer.
Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown in the greenhouse. Spikes at 20 DAP and seeds at 30 to 45 DAP were harvested, total RNA and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors ).
Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown in the greenhouse. Spikes at 20 DAP and seeds at 30 to 45 DAP were harvested, total RNA and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA Homology
Ta.27713.2.A1_x_at801WHE2AFFY
[ Show all 29 ]
DNA
tgagtgaagtctgtggagatgcaccacgagtctctcctggaggcgcttcc
tggtgataatgtcggcttcaacgtcaagaatgtggctgtcaaggatctca
agcgtgggtacgtggcctctaactccaaggacgaccccgccaaggaggct
gccaacttcaccgcccaggtcatcatcatgaaccaccccggccagatcag
caacggctacgccccggtgctggactgccacacgtcccacatcgctgtca
agttcgctgagatccagaccaagatcgaccggcggtctggcaaggagatt
gaggcggcgcccaagttcctcaagaacggtgatgccgggttcgtcaagat
gatccccaccaagcccatggtggtggagaccttcgcccagtaccctcccc
tgggtcgctttgctgtgcgtgacatgaggcagacggtcgccgttggcgtg
atcaaaagcgtggagaagaaggatccgaccggcgccaag

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