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GrainGenes Sequence Report: BE606438

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Sequence
BE606438
Contig
Ta.9542.1.S1_x_at
NSFT03P2_Contig17493
Tracefile
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External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
TIGR Gene Index
TC293300
wEST map position
BE606438
NCBI UniGene
Ta.54633
DB Remark
Locus Source: Triticum aestivum (bread wheat)
UniGene title 'Transcribed locus, moderately similar to NP_001060636.1 Os07g0678600 [Oryza sativa (japonica cultivar-group)]'
Keyword
EST
Germplasm
Chinese Spring
Species
Triticum aestivum
Cultivar
Chinese Spring
Chromosome
2AS
2BS
2DS
Clone Library
Wheat 5-15 DAP spike cDNA library
Tissue
Spike
Developmental Stage
Adult plant
Data Source
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
WHE0908_H12_P24ZS Wheat 5-15 DAP spike cDNA library Triticum aestivum cDNA clone WHE0908_H12_P24, mRNA sequence.
Other Name
WHE0908_H12_P24ZS  [ wEST-mySQL (obsolete) ]
Strain
lab_host E. coli SOLR
DNA Library
TA018E1X
Clone
WHE0908_H12_P24
Probe
WHE0908_H12_P24
Remark
DB_xref: taxon:4565
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: Stratagene SK primer.
Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown in the greenhouse. Spikes at 5, 10 and 15 DAP were harvested, total RNA and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors ).
Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown in the greenhouse. Spikes at 5, 10 and 15 DAP were harvested, total RNA and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA Homology
Ta.9542.1.S1_x_at690WHE2AFFY
[ Show all 8 ]
DNA
ctgcaaggaagtacttccagcagctgatctgcgcagttgacttttgtcac
agcaggggcgtctatcaccgtgatttgaagccggagaaccttcttcttga
tgagaacagcaacctgaaggtttcagatttcggtctcagtaccatttctg
aatgcagaagacttgacgggttgctccgcgggtgctgcggcacgcctgct
tatgttgctcccgaagtgatcaataggggaggctatgatggagccaaggc
tgacatctggtcctgtggggtgatcctctttgtgcttttggctgggtatc
tcccgttccaggataagaatctgatgaatatgtataagaagattgggaaa
gcagaattcaaatgccccagctggttctcttcagatatccgaaggcttct
gctaaggattcttgatcctaacccc

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