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GrainGenes Sequence Report: BE605086

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Sequence
BE605086
Contig
Ta.9115.1.S1_at
Ta.9115.2.S1_a_at
NSFT03P2_Contig7588
Tracefile
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External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
TIGR Gene Index
TC291378
wEST map position
BE605086
NCBI UniGene
Ta.54573
DB Remark
Locus Source: Triticum aestivum (bread wheat)
UniGene title 'Transcribed locus, strongly similar to NP_001131857.1 hypothetical protein LOC100193235 [Zea mays]'
Keyword
EST
Germplasm
Chinese Spring
Species
Triticum aestivum
Cultivar
Chinese Spring
Chromosome
4BL
4DL
Clone Library
Wheat heat stressed spike cDNA library
Tissue
Whole spike
Developmental Stage
Spikes at 5, 10, 15 and 20 days after anthesis
Data Source
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
WHE1701-1704_C13_C13ZS Wheat heat stressed spike cDNA library Triticum aestivum cDNA clone WHE1701-1704_C13_C13, mRNA sequence.
Other Name
WHE1701-1704_C13_C13ZS  [ wEST-mySQL (obsolete) ]
Strain
lab_host E. coli SOLR
DNA Library
TA032E1X
Clone
WHE1701-1704_C13_C13
Probe
WHE1701-1704_C13_C13
Remark
DB_xref: taxon:4565
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: Stratagene SK primer.
Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Spikes at 5, 10, 15 and 20 days after anthesis were heat stressed under two conditions at Texas Tech University (D. Zhang in HT Nguyrn lab): (1) at 38 C for 4 hours and (2) 5 days of cyclic treatment of 38 C for 4 hours. Total RNA and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton, Malatrasi) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA Homology
Ta.9115.1.S1_at559WHE2AFFY
Ta.9115.1.S1_at440WHE2AFFY
DNA
cttggctacgaccccaactacggtgccaggccggtgaagcgggtgcttca
gcagtacgtggagaacgagctggccaagggtatcctcagaggcgagttca
aggacgaggacagtatctctgtggacactcaggtgacggtgccgtccaat
ggccagctcccccagcagaagctcgtcttccggaagacgaacgaggaatc
caagccggctgctgctcaagatgagaagttcctgccgacagtctgatgct
ccatatcatatttttgcagaggatctataaataagtgtagatacatataa
gggcggcgaactggtagtgtcatatctctttgggcatatcatagcagggt
tatgcagc

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