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GrainGenes Sequence Report: BE604910

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Sequence	BE604910
Contig	Ta.23142.4.S1_s_at NSFT03P2_Contig18853
Tracefile	[ Download ] [ View ]
External Databases	Data at GenBank Data at EMBL Data at DDBJ
TIGR Gene Index	TC342844
NCBI UniGene	Ta.23969
DB Remark	Locus Source: Triticum aestivum (bread wheat) UniGene title 'Transcribed locus, weakly similar to XP_453852.1 unnamed protein product [Kluyveromyces lactis]'
Keyword	EST
Germplasm	Chinese Spring
Species	Triticum aestivum
Cultivar	Chinese Spring
Clone Library	Wheat heat stressed spike cDNA library
Tissue	Whole spike
Developmental Stage	Spikes at 5, 10, 15 and 20 days after anthesis
Data Source	genbank Release 135, Apr 15 2003 genbank Updated Nov 2006
Title	WHE1713-1716_F22_F22ZS Wheat heat stressed spike cDNA library Triticum aestivum cDNA clone WHE1713-1716_F22_F22, mRNA sequence.
Other Name	WHE1713-1716_F22_F22ZS  [ wEST-mySQL (obsolete) ]
Strain	lab_host E. coli SOLR
DNA Library	TA032E1X
Clone	WHE1713-1716_F22_F22
Probe	BE604910
Remark	DB_xref: taxon:4565 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: Stratagene SK primer. Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Spikes at 5, 10, 15 and 20 days after anthesis were heat stressed under two conditions at Texas Tech University (D. Zhang in HT Nguyrn lab): (1) at 38 C for 4 hours and (2) 5 days of cyclic treatment of 38 C for 4 hours. Total RNA and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton, Malatrasi) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA	agaacaaacattatctaacacaaatcaaccatgaagaaattcctcgtcct tgccctcattgttgtcgcggcgaccaccacggccgccgaaccctttactg cattccgtagtgcttgggagccgcaacatccatcatctccggagcatcaa ccaactccgcagccacaagaacatccagttccacatcaaaagttgaaccc atgcaggggggccctcctacaacagtgtagcccagttgctgatatgtcat tcctccggtcacaggtggtgcaacatagcagctgcttggtaatgtgggag cagtgctgtcancaactaaaagcgatccccaagcagtcaagatgcgaggc catccacaacgtcgtgcacgccattattttgcaacaacaacaacaattgg tacaagctacttccacccaacctcagcagcagcaacaacaaggtcaacaa caacaacagggtctgggttctagccagccacaacaacaaacacaactgga tcacggttggattgcggtgatagggacatgggtga

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