GrainGenes Sequence Report: Ta.10081.2.S1

GrainGenes Sequence Report: Ta.10081.2.S1_at

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Sequence

BE604066

Contig

Ta.10081.2.S1_at

NSFT03P2_Contig10247

Tracefile

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External Databases

TIGR Gene Index

NCBI UniGene

DB Remark

Locus Source: Triticum aestivum (bread wheat)

UniGene title 'Transcribed locus, strongly similar to NP_001058874.1 Os07g0142900 [Oryza sativa (japonica cultivar-group)]'

Keyword

EST

Germplasm

TAM W-101

Species

Triticum aestivum

Cultivar

TAM W101

TAM W-101

Clone Library

Wheat drought stressed leaf cDNA library

Tissue

Leaf

Developmental Stage

Full tillering stage

Data Source

genbank	Release 135, Apr 15 2003
genbank	Updated Nov 2006

Title

WHE1401-1404_K07_K07ZS Wheat drought stressed leaf cDNA library Triticum aestivum cDNA clone WHE1401-1404_K07_K07, mRNA sequence.

Other Name

WHE1401-1404_K07_K07ZS [ wEST-mySQL (obsolete) ]

Strain

lab_host E. coli SOLR

DNA Library

TA027E1X

Clone

WHE1401-1404_K07_K07

Remark

DB_xref: taxon:4565

Feature: source: mol_type = 'mRNA';

Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: Stratagene SK primer.

Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were given a gradual stress down to 65% and 78% RWC at Texas Tech University (D. Zhang in HT Nguyen lab). Total RNA and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close), at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).

DNA

actagctggagcttgcaactagctaggaatggcttcgctgcaggtcggcg
ccggccgcggcgtgggttgctgcttcggcccgctggacggccggcggcag
gagaggagagcgatggcggcgctgcgaccgccgagggcgggtgcgggggc
gaaggtggcggagggggacaaggtgagactgggcgagtccagcgtagcgg
tgagcaagctcggcatcggcgcctggtcgtggggcgacaccacctactgg
aacgattccgagtgggatgacaggagactgaaggaggctaaagcagcatt
cgacgccagcatcgacagcggaatgactttctttgacaccgccgaagtat
acggcacagcgctcatgggagcagtgaattcagaaagcctactgggagat
ttcatcagggagaggcagcagaagggggcggtggaggtgaccgtggccac
caa


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