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GrainGenes Sequence Report: Ta.5522.2.S1_a_at

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Sequence
BE604047
Contig
Ta.5522.1.S1_at
Ta.5522.2.S1_a_at
NSFT03P2_Contig16007
Tracefile
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External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
TIGR Gene Index
TC298591
wEST map position
BE604047
NCBI UniGene
Ta.55180
DB Remark
Locus Source: Triticum aestivum (bread wheat)
UniGene title 'Transcribed locus, strongly similar to NP_001061444.1 Os08g0282000 [Oryza sativa (japonica cultivar-group)]'
Keyword
EST
Germplasm
TAM W-101
Species
Triticum aestivum
Cultivar
TAM W101
TAM W-101
Chromosome
1BS
1DS
Clone Library
Wheat drought stressed leaf cDNA library
Tissue
Leaf
Developmental Stage
Full tillering stage
Data Source
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
WHE1401-1404_O01_O01ZS Wheat drought stressed leaf cDNA library Triticum aestivum cDNA clone WHE1401-1404_O01_O01, mRNA sequence.
Other Name
WHE1401-1404_O01_O01ZS  [ wEST-mySQL (obsolete) ]
Strain
lab_host E. coli SOLR
DNA Library
TA027E1X
Clone
WHE1401-1404_O01_O01
Probe
WHE1401-1404_O01_O01
Remark
DB_xref: taxon:4565
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: Stratagene SK primer.
Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were given a gradual stress down to 65% and 78% RWC at Texas Tech University (D. Zhang in HT Nguyen lab). Total RNA and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close), at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA Homology
Ta.5522.1.S1_at729WHE2AFFY
Ta.5522.1.S1_at48WHE2AFFY
Ta.5522.2.S1_a_at561WHE2AFFY
Ta.5522.2.S1_a_at442WHE2AFFY
Ta.5522.2.S1_x_at561WHE2AFFY
DNA
aatcaatttacagattgtgcctggattgtttcgagatggcggcgtgccgg
ggattcttcgagtgcctgctcaggctgctcaactttttcctcaccgtcgc
cggccttgccgtggtcggctacgggatctacctgctcgtggagtggatga
ggatctccggcggtggcggaggggcaccgccgtctccggccccgccggct
gagttgcttacgttcggccgcccgatgctcacggtcgtggcccttggaga
aggaggcagtttcttcgacaagctacccaaagcatggttcatctatttgt
tcattggtgttggtgctgttatcttcntcgtttctctatttggctgcatt
ggagcagggacaaggaatacgtgtcgcttgtgttgccattctttcttggc
catattattaatccttgctg

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