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GrainGenes Sequence Report: BE592051

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Sequence
BE592051
Contig
Ta.1425.1.S1_a_at
NSFT03P2_Contig13428
Tracefile
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External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
TIGR Gene Index
TC298126
NCBI UniGene
Ta.1425
DB Remark
Locus Source: Triticum aestivum (bread wheat)
UniGene title 'Transcribed locus, moderately similar to NP_001049658.1 Os03g0266700 [Oryza sativa (japonica cultivar-group)]'
Keyword
EST
Germplasm
Chinese Spring
Species
Triticum aestivum
Cultivar
Chinese Spring
Clone Library
Wheat heat stressed flag leaf cDNA library
Tissue
Flag leaf
Developmental Stage
Full tillering stage
Data Source
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
WHE1651-1654_B15_B15ZS Wheat heat stressed flag leaf cDNA library Triticum aestivum cDNA clone WHE1651-1654_B15_B15, mRNA sequence.
Other Name
WHE1651-1654_B15_B15ZS  [ wEST-mySQL (obsolete) ]
Strain
lab_host E. coli SOLR
DNA Library
TA031E1X
Clone
WHE1651-1654_B15_B15
Remark
DB_xref: taxon:4565
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: Stratagene SK primer.
Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants at full-tillering stage were heat stressed under four conditions: (1) 38 C for 2 hours, (2) 38 C for 4 hours, (3) 2 days of cyclic treatment of 38 C for 2 hours, (4) 2 days of cyclic treatment of 38 C for 4 hours, at Texas Tech University (D Zhang in Nguyen lab). Total RNA and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton, Malatrasi) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA
attatgttggggaggtaatccatccgtttgatggtcaggctgatggtgag
ctcagtatctccgtgggcgatttcgttgtggtccgccaggtgtctccaaa
cgggtggtcggaaggggagtgcaaaggaaaggcaggctggttcccatctg
cgtacgtagaggagagggacaaggccccggcaagcaaggtgatagagcct
ggacgtctgactgcgtnagcggtgctttcttctcttgtactaaatggctg
ctgtccatcgtcctgctttctcccctgtacatgatatgaggtacttgttc
aagactgctcancattttgtagaacttttttatacttacctttctgtgca
gattcttgtgcttatgtaaaagacatagtagtagcattttgcctgttaaa
aaaaaaa

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