GrainGenes Sequence Report: BE591917

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Sequence

BE591917

Tracefile

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External Databases

TIGR Gene Index

DB Remark

Locus Source: Triticum aestivum (bread wheat)

Keyword

EST

Germplasm

Chinese Spring

Species

Triticum aestivum

Cultivar

Chinese Spring

Clone Library

Wheat heat stressed flag leaf cDNA library

Tissue

Flag leaf

Developmental Stage

Full tillering stage

Data Source

genbank	Release 135, Apr 15 2003
genbank	Updated Nov 2006

Title

WHE1651-1654_K17_K17ZS Wheat heat stressed flag leaf cDNA library Triticum aestivum cDNA clone WHE1651-1654_K17_K17, mRNA sequence.

Other Name

WHE1651-1654_K17_K17ZS [ wEST-mySQL (obsolete) ]

Strain

lab_host E. coli SOLR

DNA Library

TA031E1X

Clone

WHE1651-1654_K17_K17

Probe

[Wcl531ct1319cn1832]

{SpCl-937}

BE591917

Remark

DB_xref: taxon:4565

Feature: source: mol_type = 'mRNA';

Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: Stratagene SK primer.

Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants at full-tillering stage were heat stressed under four conditions: (1) 38 C for 2 hours, (2) 38 C for 4 hours, (3) 2 days of cyclic treatment of 38 C for 2 hours, (4) 2 days of cyclic treatment of 38 C for 4 hours, at Texas Tech University (D Zhang in Nguyen lab). Total RNA and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton, Malatrasi) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).

DNA

cattaatctaaccggacacaaataaaccaacccacacctctctctgcctg
cccatttcctctctccctcttcctcccctcgccgtgggccccacccgtca
gcctaccgcgtagtagagactagagagagagagcgacaagggagggaggg
agggagggggctancagcagctagagagagaagagaaggaaagggcgccg
gaggaaggagatgggagaggagagaaaggc


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