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GrainGenes Sequence Report: BE591869

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Sequence
BE591869
Tracefile
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External Databases
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wEST map position
BE591869
DB Remark
Locus Source: Triticum aestivum (bread wheat)
Keyword
EST
Germplasm
Chinese Spring
Species
Triticum aestivum
Cultivar
Chinese Spring
Chromosome
4DS
Clone Library
Wheat heat stressed flag leaf cDNA library
Tissue
Flag leaf
Developmental Stage
Full tillering stage
Data Source
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
WHE1663-1666_F17_F17ZS Wheat heat stressed flag leaf cDNA library Triticum aestivum cDNA clone WHE1663-1666_F17_F17, mRNA sequence.
Other Name
WHE1663-1666_F17_F17ZS  [ wEST-mySQL (obsolete) ]
Strain
lab_host E. coli SOLR
DNA Library
TA031E1X
Clone
WHE1663-1666_F17_F17
Probe
BE591869
WHE1663-1666_F17_F17
Remark
DB_xref: taxon:4565
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: Stratagene SK primer.
Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants at full-tillering stage were heat stressed under four conditions: (1) 38 C for 2 hours, (2) 38 C for 4 hours, (3) 2 days of cyclic treatment of 38 C for 2 hours, (4) 2 days of cyclic treatment of 38 C for 4 hours, at Texas Tech University (D Zhang in Nguyen lab). Total RNA and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton, Malatrasi) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA Homology
Ta.12258.1.A1_at54WHE2AFFY
DNA
ttgttcactctggattttttgggcacaaccatcaagcaagctaggacgta
ttgtttaactgaagcaaagggcaggtaagctatgaatgcttcattcttcg
ttctgaacaaatgcagctactatttgtatttgatctgagcaaaggtgcaa
ccaattgaagttattttgaagaacctgcacctgagtctatatgttctgat
tttatagcctgcagagtctgtagagtaaagtttgtacattttgtgaatcg
ctttgttccggtttcccctggtggtctatactgtatcaggttatttacta
tgtgcagatttgtttgtgccattgccgatcatgtacttctttctgtgctt
ggaagcatagtctcatcgtatcccctagatctgtataagtatatttagct
cacatacacttggcatttgttcttcaaaaggcgccactccgtgtcctctc
gccctgcacgatct

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