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GrainGenes Sequence Report: BE591790

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Sequence
BE591790
Tracefile
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External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
TIGR Gene Index
TC367191
wEST map position
BE591790
NCBI UniGene
Ta.50556
DB Remark
Locus Source: Triticum aestivum (bread wheat)
UniGene title 'Transcribed locus'
Keyword
EST
Germplasm
Chinese Spring
Species
Triticum aestivum
Cultivar
Chinese Spring
Chromosome
1DS
5DL
6BS
Clone Library
Wheat heat stressed flag leaf cDNA library
Tissue
Flag leaf
Developmental Stage
Full tillering stage
Data Source
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
WHE1663-1666_G20_G20ZS Wheat heat stressed flag leaf cDNA library Triticum aestivum cDNA clone WHE1663-1666_G20_G20, mRNA sequence.
Other Name
WHE1663-1666_G20_G20ZS  [ wEST-mySQL (obsolete) ]
Strain
lab_host E. coli SOLR
DNA Library
TA031E1X
Clone
WHE1663-1666_G20_G20
Probe
WHE1663-1666_G20_G20
Remark
DB_xref: taxon:4565
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: Stratagene SK primer.
Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants at full-tillering stage were heat stressed under four conditions: (1) 38 C for 2 hours, (2) 38 C for 4 hours, (3) 2 days of cyclic treatment of 38 C for 2 hours, (4) 2 days of cyclic treatment of 38 C for 4 hours, at Texas Tech University (D Zhang in Nguyen lab). Total RNA and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton, Malatrasi) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA Homology
TaAffx.118967.1.S1_at68WHE2AFFY
Ta.3333.1.A1_at50WHE2AFFY
DNA
aaaaaggaggatgctgggatggagttacttctcgacgccgtcgacaggtt
cccactgctgtaggagttcgcggacagcgttgagcagccagatgtggctc
ctctggtgttgatgtggccaagcgaggccgcctgcggtgggactgtgaac
tcaaagggtgcttctggatgcagccagcaggtcancgatgacgagctaat
cgggaggcatggtggagctcgctgccgtgcggatgcatctccgactaggt
cggcgcagtccactgacagagtgaatccggaagttcctggatggacaaaa
agattatggctaaggagtgcgccaccagacaggacaccatgtctgtcacg
gatgagcgcgctcgaggagtcgatccgcaagtttggcgagtactgagttc
agattgtgtatgagcttatgggttttaaaatatgactgacttactttctg
caattcttgggaga

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