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GrainGenes Sequence Report: BE591521

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Sequence
BE591521
Contig
NSFT03P2_Contig8293
Tracefile
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External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
TIGR Gene Index
TC337847
wEST map position
BE591521
NCBI UniGene
Ta.48560
DB Remark
Locus Source: Triticum aestivum (bread wheat)
UniGene title 'Clone wle1n.pk0042.f7:fis, full insert mRNA sequence'
Keyword
EST
Germplasm
Chinese Spring
Species
Triticum aestivum
Cultivar
Chinese Spring
Chromosome
4AS
4BL
4DL
Clone Library
Wheat heat stressed flag leaf cDNA library
Tissue
Flag leaf
Developmental Stage
Full tillering stage
Data Source
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
WHE1659-1662_I20_I20ZS Wheat heat stressed flag leaf cDNA library Triticum aestivum cDNA clone WHE1659-1662_I20_I20, mRNA sequence.
Other Name
WHE1659-1662_I20_I20ZS  [ wEST-mySQL (obsolete) ]
Strain
lab_host E. coli SOLR
DNA Library
TA031E1X
Clone
WHE1659-1662_I20_I20
Probe
WHE1659-1662_I20_I20
Remark
DB_xref: taxon:4565
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: Stratagene SK primer.
Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants at full-tillering stage were heat stressed under four conditions: (1) 38 C for 2 hours, (2) 38 C for 4 hours, (3) 2 days of cyclic treatment of 38 C for 2 hours, (4) 2 days of cyclic treatment of 38 C for 4 hours, at Texas Tech University (D Zhang in Nguyen lab). Total RNA and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton, Malatrasi) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA Homology
Ta.494.1.S1_at337WHE2AFFY
Ta.494.1.S1_at321WHE2AFFY
DNA
aatgtagatactgaccgtgacttcttcatgagcgcgaaagaggcgaagga
gtatggccttatcgacggagtaattgtgaatcctcttaaagcacttcaac
cacttccagcttccagttagtcatgtcgtgcacaatctctgccgctccaa
gcatttttgttgtgatcttctggagctgtgtttgtaccacgttgttttcg
ttagtctggctagatgctgtaattttgcatcatgaagcttcacaggttgt
gcagaacagatgcactactagaatattcatcatttgcggttttctgcgga
tccaacaggggcacctgtactataactattcttgatgaagcacactgctg
tttatgataaactcaccgctggttgctggaaagcagatagtgcacagcaa
agcgcccagttttgagctcaaagagtgttatcaggcaacgtgaagtatgt
tccttcactgtattgtaacagggttgcacatcttttgctttatcttagcc
agcatcaagcttcccctgccaaaaaaaaaaaaaaaaaaa

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