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GrainGenes Sequence Report: BE591290

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Sequence	BE591290
Contig	Ta.12694.1.S1_at NSFT03P2_Contig11382
Tracefile	[ Download ] [ View ]
External Databases	Data at GenBank Data at EMBL Data at DDBJ
TIGR Gene Index	TC328680
wEST map position	BE591290
DB Remark	Locus Source: Triticum aestivum (bread wheat)
Keyword	EST
Germplasm	Chinese Spring
Species	Triticum aestivum
Cultivar	Chinese Spring
Chromosome	1AL 1BL 1DL
Clone Library	Wheat heat stressed flag leaf cDNA library
Tissue	Flag leaf
Developmental Stage	Full tillering stage
Data Source	genbank Release 135, Apr 15 2003 genbank Updated Nov 2006
Title	WHE1655-1658_G16_G16ZS Wheat heat stressed flag leaf cDNA library Triticum aestivum cDNA clone WHE1655-1658_G16_G16, mRNA sequence.
Other Name	WHE1655-1658_G16_G16ZS  [ wEST-mySQL (obsolete) ]
Strain	lab_host E. coli SOLR
DNA Library	TA031E1X
Clone	WHE1655-1658_G16_G16
Probe	WHE1655-1658_G16_G16
Remark	DB_xref: taxon:4565 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: Stratagene SK primer. Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants at full-tillering stage were heat stressed under four conditions: (1) 38 C for 2 hours, (2) 38 C for 4 hours, (3) 2 days of cyclic treatment of 38 C for 2 hours, (4) 2 days of cyclic treatment of 38 C for 4 hours, at Texas Tech University (D Zhang in Nguyen lab). Total RNA and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton, Malatrasi) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA Homology	Ta.12694.1.S1_at 946 WHE2AFFY
DNA	acgacctcggcctgccctccaacttccacgacaccatctgcctcaggtac ccgcagtacttccgcgtcgtcaggatggacagagggcccgcgctggagct cacggaatgggaccccgagctggccgtgtccgccgccgagatggcagagg aggagaatcgggccagggaggccgaggagaggaatttgataattgaccgg ccgctcaggttcaaccgtgtgaagctgcccaaggggctgaaggtgtcgcg gggagaggcgcggaggatcgagaaattcagagaaatgccgtacatttcgc cttacgccgacttctcgcacctgccgtcggggtcccctgagaaggagaag catgcctgtggggtggttcatgagattctaagcctgacacttgagaagcg cacgctggttgaccatctgactcatttccgggaggagttccggttctcgc agtctctgcggggaatgctcattcgccaccctgacatgttctatgtgtca ctcaagggagatagggactccgtgttcctccgtgaggcgtacaagaactc gcagctgatcgaga

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