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GrainGenes Sequence Report: BE591273

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Sequence
BE591273
Contig
Ta.12738.1.S1_at
NSFT03P2_Contig15049
Tracefile
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External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
TIGR Gene Index
TC297975
wEST map position
BE591273
NCBI UniGene
Ta.12738
DB Remark
Locus Source: Triticum aestivum (bread wheat)
UniGene title 'Transcribed locus, moderately similar to NP_001058077.1 Os06g0614300 [Oryza sativa (japonica cultivar-group)]'
Keyword
EST
Germplasm
Chinese Spring
Species
Triticum aestivum
Cultivar
Chinese Spring
Chromosome
7AL
7BL
7DL
Clone Library
Wheat heat stressed flag leaf cDNA library
Tissue
Flag leaf
Developmental Stage
Full tillering stage
Data Source
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
WHE1655-1658_K15_K15ZS Wheat heat stressed flag leaf cDNA library Triticum aestivum cDNA clone WHE1655-1658_K15_K15, mRNA sequence.
Other Name
WHE1655-1658_K15_K15ZS  [ wEST-mySQL (obsolete) ]
Strain
lab_host E. coli SOLR
DNA Library
TA031E1X
Clone
WHE1655-1658_K15_K15
Probe
WHE1655-1658_K15_K15
Remark
DB_xref: taxon:4565
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: Stratagene SK primer.
Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants at full-tillering stage were heat stressed under four conditions: (1) 38 C for 2 hours, (2) 38 C for 4 hours, (3) 2 days of cyclic treatment of 38 C for 2 hours, (4) 2 days of cyclic treatment of 38 C for 4 hours, at Texas Tech University (D Zhang in Nguyen lab). Total RNA and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton, Malatrasi) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA Homology
Ta.12738.1.S1_at991WHE2AFFY
Ta.12738.1.S1_at809WHE2AFFY
Ta.16713.2.S1_a_at54WHE2AFFY
Ta.16713.2.S1_at54WHE2AFFY
DNA
attatctcatcctgcagggtttctgatgtccaagccgagtctgccacttg
tctagatcctgatctattcacagacttcatgattcctagttggtttgagg
cagaaggtcctaccaaatacaatccatttacgttacctgaagtttactgg
gcatcacagaggaaaaagaatgcttccctggaagacattcaaaagaatat
acgagagctggagcttgatgataaccggaaaaaagaattggcatgtgccc
ttcatgctcaattcaaggactggctatatgcttcaggaaatatccgccag
ctttactgtttgcaaggcgaataagcatatatgatcttcatccgtaagtg
cttaggcacacctgtgaagattgtgcatcctttgcccggtccccttccat
agtccagtcctgtccatccgaccgcatcatccacgctgacttctcgtcga
ctgagctgattcaaccatgtagtgtttgtcaatgtaaaagaaataatgta
ctgcccatcttgtctatta

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