GrainGenes Sequence Report: Ta.12924.1.S1_at
Sequence
BE591222
Contig
Ta.12924.1.S1_at Ta.12924.2.S1_a_at NSFT03P2_Contig12974
Tracefile
[ Download ] [ View ]
External Databases
Data at GenBank Data at EMBL Data at DDBJ
TIGR Gene Index
TC288256
wEST map position
BE591222
NCBI UniGene
Ta.12924
DB Remark
Locus Source: Triticum aestivum (bread wheat) UniGene title 'Transcribed locus, moderately similar to NP_001045132.1 Os01g0906200 [Oryza sativa (japonica cultivar-group)]'
Keyword
EST
Germplasm
Chinese Spring
Species
Triticum aestivum
Cultivar
Chinese Spring
Chromosome
3AL
Clone Library
Wheat heat stressed flag leaf cDNA library
Tissue
Flag leaf
Developmental Stage
Full tillering stage
Data Source
genbank Release 135, Apr 15 2003 genbank Updated Nov 2006
Title
WHE1655-1658_D12_D12ZS Wheat heat stressed flag leaf cDNA library Triticum aestivum cDNA clone WHE1655-1658_D12_D12, mRNA sequence.
Other Name
WHE1655-1658_D12_D12ZS [ wEST-mySQL (obsolete) ]
Strain
lab_host E. coli SOLR
DNA Library
TA031E1X
Clone
WHE1655-1658_D12_D12
Probe
WHE1655-1658_D12_D12
Remark
DB_xref: taxon:4565 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: Stratagene SK primer. Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants at full-tillering stage were heat stressed under four conditions: (1) 38 C for 2 hours, (2) 38 C for 4 hours, (3) 2 days of cyclic treatment of 38 C for 2 hours, (4) 2 days of cyclic treatment of 38 C for 4 hours, at Texas Tech University (D Zhang in Nguyen lab). Total RNA and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton, Malatrasi) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA Homology
Ta.12924.1.S1_at 1001 WHE2AFFY Ta.12924.1.S1_at 341 WHE2AFFY Ta.12924.2.S1_a_at 466 WHE2AFFY Ta.12924.2.S1_a_at 418 WHE2AFFY
DNA
tcggcacgaggctagtacctaccgtgcgctccttccagaagcacgcctct
tgagtttcagcggatacttgagctcagggcttgaagaaagcagaccaaca
agctatactggaagacttcaacaagcacagccccagcatcaagcacccta
ccattacccccacagccgcgccaccagttccaacagccacagcatcagtc
ccgatggcaccagtcccgctcccaacccaagccgtcgcagcgtcaccgag
catgtccaccgccttgacgggcgccctggccaaccgggaggatgtgctcg
cccgcgccgcagcgctcgggcgcggcgctgccacgacggggttcaagagg
ttcctcgcattgacagaggcggccaaagaccggaaggacgggccgttccg
taagctgttcaacgcgtaaagttttgcccctgcttgttgatagtttacgc
ctctctttgcagtgcacatagctataggaaattcttcccacaccgtgtgt
gttccttgtgtgtaccctgaccgtcagtgtataatggttagatctttttt
gacgtgacgctccccattttgc
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Sequence Report: Ta.12924.1.S1_at
|
| |||||||||||||
|
| |||||||||||||
|
| |||||||||||||
|
| |||||||||||||
|
| |||||||||||||
|
| |||||||||||||
|
| |||||||||||||
|
| |||||||||||||
|
| |||||||||||||
|
| |||||||||||||
|
| |||||||||||||
|
| |||||||||||||
|
| |||||||||||||
|
| |||||||||||||
|
| |||||||||||||
|
| |||||||||||||
|
| |||||||||||||
|
| |||||||||||||
|
| |||||||||||||
|
| |||||||||||||
|
| |||||||||||||
|
| |||||||||||||
|
| |||||||||||||
|
| |||||||||||||
|
| |||||||||||||
|
|
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||