Query (optional)   in Class  

GrainGenes Sequence Report: BE591173

[Submit comment/correction]

Sequence
BE591173
Tracefile
[ Download ]
[ View ]
External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
wEST map position
BE591173
DB Remark
Locus Source: Triticum aestivum (bread wheat)
Keyword
EST
Germplasm
Chinese Spring
Species
Triticum aestivum
Cultivar
Chinese Spring
Chromosome
6AL
6BL
Clone Library
Wheat heat stressed flag leaf cDNA library
Tissue
Flag leaf
Developmental Stage
Full tillering stage
Data Source
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
WHE1655-1658_O11_O11ZS Wheat heat stressed flag leaf cDNA library Triticum aestivum cDNA clone WHE1655-1658_O11_O11, mRNA sequence.
Other Name
WHE1655-1658_O11_O11ZS  [ wEST-mySQL (obsolete) ]
Strain
lab_host E. coli SOLR
DNA Library
TA031E1X
Clone
WHE1655-1658_O11_O11
Probe
WHE1655-1658_O11_O11
Remark
DB_xref: taxon:4565
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: Stratagene SK primer.
Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants at full-tillering stage were heat stressed under four conditions: (1) 38 C for 2 hours, (2) 38 C for 4 hours, (3) 2 days of cyclic treatment of 38 C for 2 hours, (4) 2 days of cyclic treatment of 38 C for 4 hours, at Texas Tech University (D Zhang in Nguyen lab). Total RNA and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton, Malatrasi) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA Homology
TaAffx.78864.1.S1_at88WHE2AFFY
[ Show all 13 ]
DNA
acggttagatacgagctcaaatctgtgtgcgcatccacgtacatacgcca
tggcgctccctgggcttgctttgcctaattctcagatcgtcctgtcggca
gcatgcacgggtccgcatgtaacagtcggacgaccttcaagggcacgctt
cgtgtattccttgacgtcggtgaaccgtaagctcatcgtaagctcgtcct
ctatcaataatccctacacgacagctggcggcatgcaacaagtggcggca
ggggaagacgagcatgagctaaagctcctagggacatgggaggggccctt
tcgcgatgcgagtgaggctggcgctcaacttcaagggcctgagctacgag
taccaggaagaggaccttgcaaataagagtgaccttctcctcgtgtcaaa
cccggtgaacaagaaggtgcccgtgctcatccacaatggtatgcccatat
gcgagtcactcgctatcttggagtacatcgatgaggtctaccatgggata
ggaccctctctccttcccgcccacccctaccaacgcgctagggctcgatt
ctgggccacctacattgacaacaagct

GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.