GrainGenes Sequence Report: NSFT03P2_Contig7967
Sequence
BE591000
Contig
Ta.25903.1.A1_at NSFT03P2_Contig7967
Tracefile
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External Databases
Data at GenBank Data at EMBL Data at DDBJ
TIGR Gene Index
TC288294
wEST map position
BE591000
NCBI UniGene
Ta.41190
DB Remark
Locus Source: Triticum aestivum (bread wheat) UniGene title 'Transcribed locus, moderately similar to NP_001049468.1 Os03g0232500 [Oryza sativa (japonica cultivar-group)]'
Keyword
EST
Germplasm
Chinese Spring
Species
Triticum aestivum
Cultivar
Chinese Spring
Chromosome
4DL
Clone Library
Wheat heat stressed flag leaf cDNA library
Tissue
Flag leaf
Developmental Stage
Full tillering stage
Data Source
genbank Release 135, Apr 15 2003 genbank Updated Nov 2006
Title
WHE1655-1658_N04_N04ZS Wheat heat stressed flag leaf cDNA library Triticum aestivum cDNA clone WHE1655-1658_N04_N04, mRNA sequence.
Other Name
WHE1655-1658_N04_N04ZS [ wEST-mySQL (obsolete) ]
Strain
lab_host E. coli SOLR
DNA Library
TA031E1X
Clone
WHE1655-1658_N04_N04
Probe
WHE1655-1658_N04_N04
Remark
DB_xref: taxon:4565 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: Stratagene SK primer. Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants at full-tillering stage were heat stressed under four conditions: (1) 38 C for 2 hours, (2) 38 C for 4 hours, (3) 2 days of cyclic treatment of 38 C for 2 hours, (4) 2 days of cyclic treatment of 38 C for 4 hours, at Texas Tech University (D Zhang in Nguyen lab). Total RNA and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton, Malatrasi) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA Homology
Ta.25903.1.A1_at 936 WHE2AFFY Ta.25903.1.A1_at 662 WHE2AFFY TaAffx.77659.1.S1_at 98 WHE2AFFY TaAffx.77659.1.S1_at 82 WHE2AFFY
DNA
gactcaaacaattaaccatcatttgattaacaaaagttggtaccttgtgg
atttgcctggttacgggtttgcagctgcgtccaagacagctcgaacggat
tggtcttcatttaccaaggggtatttcttgaacagggacactttggtcgg
tgtactgctccttgttgatgctagtgttccaactcaacaaattgaccttg
actgtgctaactggcttggtcgtaacaatgtcgggctaacctttgtgttc
accaagtgtgacaaagtaaagaaaggcaaagggggtcggcctgatgagaa
catcaaagtgttcctggagaacattggctcgctctatccaaagcctcctc
catggatcatgacgagcagcaccacaggactaggccgtgacggactgctc
ctccatatgtcgcagttgaggaactactgggacaatgaggcaacctaagt
ccggggttcttctacaggctcgagacagctgaag
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Sequence Report: NSFT03P2_Contig7967
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