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GrainGenes Sequence Report: NSFT03P2_Contig14029

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Sequence
BE587932
Contig
NSFT03P2_Contig14029
Tracefile
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External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
DB Remark
Locus Source: Secale cereale (rye)
Keyword
EST
Germplasm
Blanco
Species
Secale cereale
Cultivar
Blanco
Clone Library
Secale cereale root tip cDNA library
Tissue
Root tip
Developmental Stage
Seedlings
Data Source
genbankRelease 135, Apr 15 2003
genbankDownloaded 2008-2009
Title
WHE0665_E11_J21ZM Secale cereale root tip cDNA library Secale cereale cDNA clone WHE0665_E11_J21, mRNA sequence.
Other Name
WHE0665_E11_J21ZM  [ wEST-mySQL (obsolete) ]
Strain
lab_host E. coli DH12S
DNA Library
SC013XXX
Clone
WHE0665_E11_J21
Probe
SCM81
Remark
DB_xref: taxon:4550
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: M13 forward primer.
Note: Vector: pSPORT2; Site_1: SalI; Site_2: NotI; Seeds were germinated aseptically on filter paper and transferred to a hydroponic growth system in a growth chamber when the primary root was 1 cm in length. Plants were grown in an environmental chamber until harvesting. The tissue, total RNA, and poly(A) RNA were prepared and a cDNA library was made (Butler and Gustafson) at University of Missouri, Columbia. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA
ccacgcgtccgaatcaaaaccctaagcaagtcggcggcggcggcggcgtc
agcgcaagcatcccctactcaccccaggtccagcctctcccctcccatcc
ttccccaacatgctcccccagatgcgccccctcccttcccgcccagccat
ccacgccgtccaccggcgcacctcccccgcgcctccctctatcggcggac
ttgggcagcatggccgagtggccctcgtgaggaagacgacgcccgtggta
tgccgctccgtcgccagcccggcagccaatcagggggcgccagcggtgga
gagaccttggaagcttagcgacgctcgcctcgttctcgaggacggctccg
tgtggaatgccaaatccttcggtgcttccgggactcaagtcggcgaggtg
gttttcaatacctcattgacagggta

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