GrainGenes Sequence Report: BE587793

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Sequence

BE587793

Tracefile

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External Databases

Data at GenBank

Data at EMBL

Data at DDBJ

DB Remark

Locus Source: Secale cereale (rye)

Keyword

EST

Germplasm

Blanco

Species

Secale cereale

Cultivar

Blanco

Clone Library

Secale cereale root tip cDNA library

Tissue

Root tip

Developmental Stage

Seedlings

Data Source

genbank	Release 135, Apr 15 2003
genbank	Downloaded 2008-2009

Title

WHE0663_E05_I09ZM Secale cereale root tip cDNA library Secale cereale cDNA clone WHE0663_E05_I09, mRNA sequence.

Other Name

WHE0663_E05_I09ZM [ wEST-mySQL (obsolete) ]

Strain

lab_host E. coli DH12S

DNA Library

SC013XXX

Clone

WHE0663_E05_I09

Probe

SCM100

Remark

DB_xref: taxon:4550

Feature: source: mol_type = 'mRNA';

Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: M13 forward primer.

Note: Vector: pSPORT2; Site_1: SalI; Site_2: NotI; Seeds were germinated aseptically on filter paper and transferred to a hydroponic growth system in a growth chamber when the primary root was 1 cm in length. Plants were grown in an environmental chamber until harvesting. The tissue, total RNA, and poly(A) RNA were prepared and a cDNA library was made (Butler and Gustafson) at University of Missouri, Columbia. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).

DNA

ccacgcgtccggaaacaacgtgtgttcttcttcttcttcttcctcctcct
cctcctcctccaagctccacgcttccacagcatccctagaaaagaaacgc
tcctctccctcctctcctcgcagagttttccgaggaagaaatcccagtcc
aaagagcttgcctatcgccaaggaaccgagaggaaacgcagatacgtatc
gcttccattccatctgcgtgagaggatgatcccgtttatagagcagagga
gctccagttgatctagccgtgctggccctcgagcggaagcccatcgatgg
cgatgacggaagcgcatggcggcacgccgaggaccgacgccggcgggagc
agggtggagtccgagcagaggaagctgcggcgcgtcgagctcccctcctg
gtcctgccctcccaacc


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