GrainGenes Sequence Report: BE586813

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Sequence

BE586813

Tracefile

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External Databases

Data at GenBank

Data at EMBL

Data at DDBJ

DB Remark

Locus Source: Secale cereale (rye)

Keyword

EST

Germplasm

Blanco

Species

Secale cereale

Cultivar

Blanco

Clone Library

Secale cereale aluminum-stressed root tip cDNA library

Tissue

Root tip

Developmental Stage

Seedling

Data Source

genbank	Release 135, Apr 15 2003
genbank	Downloaded 2008-2009

Title

WHE0508_H08_P16ZR Secale cereale aluminum-stressed root tip cDNA library Secale cereale cDNA clone WHE0508_H08_P16, mRNA sequence.

Other Name

WHE0508_H08_P16ZR [ wEST-mySQL (obsolete) ]

Strain

lab_host E. coli DH12S

DNA Library

SC010XXX

Clone

WHE0508_H08_P16

Probe

SCM133

REMS1205

Remark

DB_xref: taxon:4550

Feature: source: mol_type = 'mRNA';

Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: M13 reversed primer.

Note: Vector: pSPORT1; Site_1: SalI; Site_2: NotI; Seeds were germinated aseptically on filter paper and transferred to a hydroponic growth system in a growth chamber when the primary root was 1 cm in length. After a 2-day establishment period, seedlings were subjected to a 5 ppm aluminum stress prior to tissue harvest. Plants were grown in an environmental chamber. The tissue, total RNA, and poly(A) RNA were prepared, and a cDNA library was made (Butler and Gustafson) at University of Missouri , Columbia. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors ).

Note: Vector: pSPORT1; Site_1: SalI; Site_2: NotI; Seeds were germinated aseptically on filter paper and transferred to a hydroponic growth system in a growth chamber when the primary root was 1 cm in length. After a 2-day establishment period, seedlings were subjected to a 5 ppm aluminum stress prior to tissue harvest. Plants were grown in an environmental chamber. The tissue, total RNA, and poly(A) RNA were prepared, and a cDNA library was made (Butler and Gustafson) at University of Missouri, Columbia. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).

DNA

ccacgcgtgcggaagaccagagaggagatgtcaccgttcatccatcgttc
cttgttttgccaaagaaggctttggtggcacatacatacatacatacata
catttggaagtccaaatttcaagtcatcatctggtttatattcctggtgt
tgctcagatatcatgtgagttataatagcatccagagctgtatatacata
cacaaagaaaacgagacatgcttatgcatgagtatgctttgtaactctgc
atctggaaacccgggagcaacattggttggttcctccatgatgtgatcac
ttgaaattgatgatttgatttgatttttttatatatatttgttgggttgg
cgttgtttnaaaaaaaaaaaaaaaaaaaaaaaaaaag


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