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GrainGenes Sequence Report: BE586531

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Sequence	BE586531
Contig	NSFT03P2_Contig14327
Tracefile	[ Download ] [ View ]
External Databases	Data at GenBank Data at EMBL Data at DDBJ
TIGR Gene Index	TC4862
DB Remark	Locus Source: Secale cereale (rye)
Keyword	EST
Germplasm	Blanco
Species	Secale cereale
Cultivar	Blanco
Clone Library	Secale cereale aluminum-stressed root tip cDNA library
Tissue	Root tip
Developmental Stage	Seedling
Data Source	genbank Release 135, Apr 15 2003 genbank Downloaded 2008-2009
Title	WHE0506_A04_A08ZR Secale cereale aluminum-stressed root tip cDNA library Secale cereale cDNA clone WHE0506_A04_A08, mRNA sequence.
Other Name	WHE0506_A04_A08ZR  [ wEST-mySQL (obsolete) ]
Strain	lab_host E. coli DH12S
DNA Library	SC010XXX
Clone	WHE0506_A04_A08
Probe	SCM149
Remark	DB_xref: taxon:4550 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: M13 reversed primer. Note: Vector: pSPORT1; Site_1: SalI; Site_2: NotI; Seeds were germinated aseptically on filter paper and transferred to a hydroponic growth system in a growth chamber when the primary root was 1 cm in length. After a 2-day establishment period, seedlings were subjected to a 5 ppm aluminum stress prior to tissue harvest. Plants were grown in an environmental chamber. The tissue, total RNA, and poly(A) RNA were prepared, and a cDNA library was made (Butler and Gustafson) at University of Missouri , Columbia. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors ). Note: Vector: pSPORT1; Site_1: SalI; Site_2: NotI; Seeds were germinated aseptically on filter paper and transferred to a hydroponic growth system in a growth chamber when the primary root was 1 cm in length. After a 2-day establishment period, seedlings were subjected to a 5 ppm aluminum stress prior to tissue harvest. Plants were grown in an environmental chamber. The tissue, total RNA, and poly(A) RNA were prepared, and a cDNA library was made (Butler and Gustafson) at University of Missouri, Columbia. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA	ccacgcgtccgcaaggagtgccggcacgagcagatgcagctcgacgccgt ctccgccaggcaggccgcccggaggctgcagcggttctcggcgggagcag agtccgggtgtgcacaccacggggtgtccgtctcgagctaaccggcggga gtgccaagccacaaacacgatctccgtgcaccaagccgctggtgtccgtg gttggctgatcgagcagcgcgaggtcgaggaaacttgcctagcttgaaga agcaaggggcgggccaccggagtcgctcacggcaggccggcgctgcatgt gccgccggtcaagtgatttgggcaaaaacgcagcggagaaggggctggat tggatctgaagaaagtcaagcaaacaagattccccacggtttaactttga ggcggtcatgtctgcccctcgttttttctttctttctgcgttattgtcac ttctgtataagctcatttgcagcggtactctcgctcggtaaactttcaag cgaatcgacatacaaaaaaaaaaaaaaaa

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