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GrainGenes Sequence Report: BE586181

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Sequence
BE586181
Contig
Ta.30739.1.A1_at
NSFT03P2_Contig9943
External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
NCBI UniGene
Ta.49600
DB Remark
Locus Source: Triticum aestivum (bread wheat)
UniGene title 'Clone wlm96.pk025.j5:fis, full insert mRNA sequence'
Keyword
EST
Species
Triticum aestivum
Cultivar
Sumai3
Clone Library
KSU wheat Fusarium graminearum infected spike cDNA library
Tissue
Spike
Developmental Stage
Adult plant
Data Source
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
Est7-4-5_B08_7E5sp6_061 KSU wheat Fusarium graminearum infected spike cDNA library Triticum aestivum cDNA clone Est7-4-5_B08_7E5sp6_061, mRNA sequence.
Strain
lab_host E. coli JM109
Clone
Est7-4-5_B08_7E5sp6_061
Probe
MAG2969
Remark
DB_xref: taxon:4565
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: John Fellers; US Department of Agriculture, Agriculture Research Service, Plant; Science and Entomology Unit; Dept. of Plant Pathology, 4006 Throckmorton Hall, Kansas State; University, Manhattan, KS 66506, USA; Tel: 785-532-2367; Fax: 785-532-6167; Email: jpf@alfalfa.ksu.edu; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: SP6.
Note: Vector: pGEM-T easy; Site_1: SacII; Site_2: SpeI; Plants were grown in the greenhouse. Spikes were sprayed with Fusarium graminearum (at what stage). Total RNA, and poly(A) RNA were prepared from infected spikes. cDNA was prepared using the SmartTM PCR cDNA synthesis kit from Clonetech. cDNA was cloned into the pGEM-T easy vector from Promega.
DNA
cggggatctcatcatctaagatagattcacagatagtacatatttccttt
ccctcaaaaaagaagaaagattatacatattccctcacttttgtaagcca
tagcgatggagtactcgctgaagctggcagtagtactgctcttagctctc
gcgtccgccatggcggtcacggcccagaactcgccgcaggacttcgtgga
cccccacaacgcggcgcgcgccgacgtcggcgtcgggccggtgacctggg
acgacaacgtggccgcgtacgcgcagaactacacggagcagcgccgtgga
aactgccagctgatacatactgccgatggccggccgaacggggagaacct
ataatgaggctgttgtaccaattggactgatgtttgtctctttttgtcta
tt

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