GrainGenes Sequence Report: NSFT03P2_Contig14276
Sequence
BE586092
Contig
NSFT03P2_Contig14276
External Databases
Data at GenBank Data at EMBL Data at DDBJ
TIGR Gene Index
TC310132
wEST map position
BE586092
NCBI UniGene
Ta.40001
DB Remark
Locus Source: Triticum aestivum (bread wheat) UniGene title 'Transcribed locus, moderately similar to NP_001060838.1 Os08g0113200 [Oryza sativa (japonica cultivar-group)]'
Keyword
EST
Species
Triticum aestivum
Cultivar
Sumai3
Chromosome
7AL 7BL 7DL
Clone Library
KSU wheat Fusarium graminearum infected spike cDNA library
Tissue
Spike
Developmental Stage
Adult plant
Data Source
genbank Release 135, Apr 15 2003 genbank Updated Nov 2006
Title
Est#8pT7_H09_h9_076 KSU wheat Fusarium graminearum infected spike cDNA library Triticum aestivum cDNA clone Est#8pT7_H09_h9_076, mRNA sequence.
Strain
lab_host E. coli JM109
Clone
Est#8pT7_H09_h9_076
Probe
Est#8pT7_H09_h9_076
Remark
DB_xref: taxon:4565 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: John Fellers; US Department of Agriculture, Agriculture Research Service, Plant; Science and Entomology Unit; Dept. of Plant Pathology, 4006 Throckmorton Hall, Kansas State; University, Manhattan, KS 66506, USA; Tel: 785-532-2367; Fax: 785-532-6167; Email: jpf@alfalfa.ksu.edu; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: T7. Note: Vector: pGEM-T easy; Site_1: SacII; Site_2: SpeI; Plants were grown in the greenhouse. Spikes were sprayed with Fusarium graminearum (at what stage). Total RNA, and poly(A) RNA were prepared from infected spikes. cDNA was prepared using the SmartTM PCR cDNA synthesis kit from Clonetech. cDNA was cloned into the pGEM-T easy vector from Promega.
DNA
gcggggaagcagtatgaggatgaactaagcgtcgcggctgaatccgaggc
taacaaactacaagtgttcctgaaatacatcaaatttgaggagtcttttg
gtgaccctgctcgtgtccaagttttatacgaacgggccgtttctgagctt
cccgtttcaagtgatctatggatgggatacacgagttacctggacagaac
cctaaaggtacctgctatactaaaaagtgtttaccacagagcgacgagaa
actgtacatgggttagtgacctatgggtccactacttgctgtctttggag
cgcattcgtgcatctgaagacgaactgcgacatgtctttgagctagccat
tcaatgttctttcccaagcatgaacgagtacctcaatatttatcttactc
gagttgatagtttaagacggagaatgccaactggcttggatttcgaactg
attaggcagacattcgtggatgctgctgagttcctctcaccacaactagg
aattgaggagctattactcttgcatgcatactgggctaaattagagtgta
atattggcaaagctatagctgcagcacgcggagtttgggaaaatgctctc
aagaaaagtgggtctgttttggaggtttggcagcactatatttcaatgga
gatagaaatggggcatactcatgaggcaagatcactttacaagncgtg
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Sequence Report: NSFT03P2_Contig14276
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||