GrainGenes Sequence Report: BE586072
Sequence
BE586072
Contig
NSFT03P2_Contig4890
External Databases
Data at GenBank Data at EMBL Data at DDBJ
TIGR Gene Index
TC334733
wEST map position
BE586072
NCBI UniGene
Ta.22400
DB Remark
Locus Source: Triticum aestivum (bread wheat) UniGene title 'Transcribed locus, moderately similar to NP_001062394.1 Os08g0542700 [Oryza sativa (japonica cultivar-group)]'
Keyword
EST
Species
Triticum aestivum
Cultivar
Sumai3
Chromosome
7BS 7DS
Clone Library
KSU wheat Fusarium graminearum infected spike cDNA library
Tissue
Spike
Developmental Stage
Adult plant
Data Source
genbank Release 135, Apr 15 2003 genbank Updated Nov 2006
Title
Est#8pT7_F03_f3_027 KSU wheat Fusarium graminearum infected spike cDNA library Triticum aestivum cDNA clone Est#8pT7_F03_f3_027, mRNA sequence.
Strain
lab_host E. coli JM109
Clone
Est#8pT7_F03_f3_027
Probe
Est#8pT7_F03_f3_027
Remark
DB_xref: taxon:4565 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: John Fellers; US Department of Agriculture, Agriculture Research Service, Plant; Science and Entomology Unit; Dept. of Plant Pathology, 4006 Throckmorton Hall, Kansas State; University, Manhattan, KS 66506, USA; Tel: 785-532-2367; Fax: 785-532-6167; Email: jpf@alfalfa.ksu.edu; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: T7. Note: Vector: pGEM-T easy; Site_1: SacII; Site_2: SpeI; Plants were grown in the greenhouse. Spikes were sprayed with Fusarium graminearum (at what stage). Total RNA, and poly(A) RNA were prepared from infected spikes. cDNA was prepared using the SmartTM PCR cDNA synthesis kit from Clonetech. cDNA was cloned into the pGEM-T easy vector from Promega.
DNA Homology
Ta.22400.1.S1_at 385 WHE2AFFY TaAffx.112784.1.S1_at 105 WHE2AFFY TaAffx.112784.1.S1_at 60 WHE2AFFY
DNA
gcggggggtcacattgatattgtcaacctcctactggaaactgatgctag
ccttgccaaaattgcgagaaataatgggaaaacagttttgcattccgcgg
cgaggatgggccatgtggaagttgtaagatccttgttaaataaggatcca
gggattggtttaagaacagacaagaaggggcagacggcgctgcatatggc
gtccaaaggaacgaatgctgaaattgtggttgagttgttgaagcctgatt
tctcagttagccatctagaagataacaaggggaacaggccactgcatgtt
gcgactcggaagggcaatatcgttatagtgcagactctactgtcagttga
ggggattgatgtcaatgcagttaacaggtctggagagactgctcttgcca
ttgctgagaagatgaacaaccaagaactcgtcaacattctgagagacgcc
ggcggcgtcgtaactgcaaaagagccagtgcatcctgcaaacccggcaaa
gcagctgaagcaaaccgtgagtgatatcaggcacgacgtccagtcccaga
tcaagcagacacggcagaccaagatgcaggtccagaagatcaagagcagg
ctggagaagctccacatcggcggcctgtacaacgccatcaactccaacac
cgtggtggcggtgctcatcgccaccgtcgccttcgctgtcatattcaccg
tgctctgcaactttt
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Sequence Report: BE586072
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||