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GrainGenes Sequence Report: TaAffx.53419.1.S1_s_at

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Sequence
BE585887
Contig
Ta.9580.1.S1_at
TaAffx.53419.1.S1_s_at
NSFT03P2_Contig11773
External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
TIGR Gene Index
TC322857
NCBI UniGene
Ta.35998
DB Remark
Locus Source: Triticum aestivum (bread wheat)
UniGene title 'Transcribed locus, moderately similar to NP_001063966.1 Os09g0567900 [Oryza sativa (japonica cultivar-group)]'
Keyword
EST
Species
Triticum aestivum
Cultivar
Sumai3
Clone Library
KSU wheat Fusarium graminearum infected spike cDNA library
Tissue
Spike
Developmental Stage
Adult plant
Data Source
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
Est#3pSP6_B06_b6_045 KSU wheat Fusarium graminearum infected spike cDNA library Triticum aestivum cDNA clone Est#3pSP6_B06_b6_045, mRNA sequence.
Strain
lab_host E. coli JM109
Clone
Est#3pSP6_B06_b6_045
Remark
DB_xref: taxon:4565
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: John Fellers; US Department of Agriculture, Agriculture Research Service, Plant; Science and Entomology Unit; Dept. of Plant Pathology, 4006 Throckmorton Hall, Kansas State; University, Manhattan, KS 66506, USA; Tel: 785-532-2367; Fax: 785-532-6167; Email: jpf@alfalfa.ksu.edu; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: SP6.
Note: Vector: pGEM-T easy; Site_1: SacII; Site_2: SpeI; Plants were grown in the greenhouse. Spikes were sprayed with Fusarium graminearum (at what stage). Total RNA, and poly(A) RNA were prepared from infected spikes. cDNA was prepared using the SmartTM PCR cDNA synthesis kit from Clonetech. cDNA was cloned into the pGEM-T easy vector from Promega.
DNA
gcaattcacaacgagcagttcaataattccacgagacaatgtgtctacat
gaagagcgtcgaatggggtacacataaatcacgagtcgatcaaccgacgg
atctctacaatttcaaatcatttgatgcgcagatatacagatcatgaact
gacgcttaatttttgaggagctccttgacgaaggcgaccaccttggcctt
gtccaccgtccacgccaccgagatgggcgtgtagcccgaccacgcgttct
ccgacttgaacttcttgagccccatgtccatggaggtgtggcctctgcag
atgccctgggtctccacccgcaccacgcccttcctgaaggtgaagagctc
cggccggaccagcgccgcgaggctcaccgggtcgtggaggaacacggcgg
ggtcgccgtacgagtggaggtgccagtcgctgtaaaacttgcagatgtcg
caaaggaactgcgcctgcttgcccccggacctcctcagctccgacaagtc
cgcctccgtgaagctcacctgggtagtgatgttgagacccaccaccgcga
tgtccgcgcccgacgtgtataccatgtccgccgcctccggatcactgagg
atgtttgcttcggctgaaggggtggcatacct

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