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GrainGenes Sequence Report: BE585782

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Sequence
BE585782
External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
NCBI UniGene
Ta.40338
DB Remark
Locus Source: Triticum aestivum (bread wheat)
UniGene title 'Transcribed locus, moderately similar to NP_001052573.1 Os04g0373400 [Oryza sativa (japonica cultivar-group)]'
Keyword
EST
Species
Triticum aestivum
Cultivar
Sumai3
Clone Library
KSU wheat Fusarium graminearum infected spike cDNA library
Tissue
Spike
Developmental Stage
Adult plant
Data Source
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
Est#1pT7_C06_c6_038 KSU wheat Fusarium graminearum infected spike cDNA library Triticum aestivum cDNA clone Est#1pT7_C06_c6_038, mRNA sequence.
Strain
lab_host E. coli JM109
Clone
Est#1pT7_C06_c6_038
Probe
MAG2922
Remark
DB_xref: taxon:4565
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: John Fellers; US Department of Agriculture, Agriculture Research Service, Plant; Science and Entomology Unit; Dept. of Plant Pathology, 4006 Throckmorton Hall, Kansas State; University, Manhattan, KS 66506, USA; Tel: 785-532-2367; Fax: 785-532-6167; Email: jpf@alfalfa.ksu.edu; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: T7.
Note: Vector: pGEM-T easy; Site_1: SacII; Site_2: SpeI; Plants were grown in the greenhouse. Spikes were sprayed with Fusarium graminearum (at what stage). Total RNA, and poly(A) RNA were prepared from infected spikes. cDNA was prepared using the SmartTM PCR cDNA synthesis kit from Clonetech. cDNA was cloned into the pGEM-T easy vector from Promega.
DNA
gcggggagagccagagctttcaagtttcaagtatgttgccagccatggag
gagccccttgttgggggcagaagcgacaccgaggagacaggagggccaaa
ggagagcttggtggtgactgaggttaagaagcagctcctacctcgccggg
cccctcatcgccggatgcctgccgcagaacgtcgtgcagatgatatcggt
catgtttgtcggccatctcggcgagctcgccctctcgagtgcctccatcg
ccacctcctttgcgaatgtcaccggcttcagcttgatggctggcatggcg
agcagcttggacacactgtgtggtcaagccttcggggcaaaacggtacca
tcagctcggcatctacaagcagagggcgatcctcgtgctcactctggtga
gtgttgtggttgcggtgctctgggcgtacaccgggcagatcctcctgctc
ttcggccaggacccagagattgccatgggggcagggagctacatccggtg
gatgattccggcactgttcgcttacggactgctgcagtgccacgtgcgat
tcctgcagacacaaagcatcgtactcccggtgatggcgagcgcaggcgtg
acggcgctgagccacgtgcttgtgtgctggatgctggtgtacaagcttgg
gctggacacaagggcgctgccctgaccaatgctgactgagacctgagcaa
tgtgtgaa

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