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GrainGenes Sequence Report: NSFT03P2_Contig3899

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Sequence
BE585744
Contig
Ta.9377.2.A1_at
Ta.9377.3.S1_a_at
NSFT03P2_Contig3899
External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
TIGR Gene Index
TC366833
wEST map position
BE585744
NCBI UniGene
Ta.55978
DB Remark
Locus Source: Triticum aestivum (bread wheat)
UniGene title 'Transcribed locus, moderately similar to NP_001062275.1 Os08g0521400 [Oryza sativa (japonica cultivar-group)]'
Keyword
EST
Species
Triticum aestivum
Cultivar
Sumai3
Chromosome
7AS
7BS
7DS
Clone Library
KSU wheat Fusarium graminearum infected spike cDNA library
Tissue
Spike
Developmental Stage
Adult plant
Data Source
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
Est#1SP6_D06_d6_046 KSU wheat Fusarium graminearum infected spike cDNA library Triticum aestivum cDNA clone Est#1SP6_D06_d6_046, mRNA sequence.
Strain
lab_host E. coli JM109
Clone
Est#1SP6_D06_d6_046
Probe
Est#1SP6_D06_d6_046
Remark
DB_xref: taxon:4565
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: John Fellers; US Department of Agriculture, Agriculture Research Service, Plant; Science and Entomology Unit; Dept. of Plant Pathology, 4006 Throckmorton Hall, Kansas State; University, Manhattan, KS 66506, USA; Tel: 785-532-2367; Fax: 785-532-6167; Email: jpf@alfalfa.ksu.edu; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: SP6.
Note: Vector: pGEM-T easy; Site_1: SacII; Site_2: SpeI; Plants were grown in the greenhouse. Spikes were sprayed with Fusarium graminearum (at what stage). Total RNA, and poly(A) RNA were prepared from infected spikes. cDNA was prepared using the SmartTM PCR cDNA synthesis kit from Clonetech. cDNA was cloned into the pGEM-T easy vector from Promega.
DNA Homology
Ta.9377.2.A1_at1247WHE2AFFY
[ Show all 9 ]
DNA
gcaaagacaaattaattccacggccgtcaaaaaagtcacctacaaaattt
agccttgggcgataacaaacaagattctggagctcagtgttcttacaaag
acagtccatcgctcgatgtcatcatcactctggctccagtgatacttcta
ttttttgctcatccacctccagggtatttaaaaaataaaatggatgccta
cagaactaactaatctctccatgggtcattctcattctcagctcctcccc
cgtatggctcaaacctctataatgggcatcccatgccacgctatggcatt
cctcagctccctccgggctctggacatccgtatggctatggcgggcgagt
cccaatggggagcccctatgggccaatgcatatggcggcgccacccccgt
attctagtggttctatggttggtgcaggtggaatgtacagcatcagcatg
cccatggatagatatggcttgggctcacccgctggtcctggggcaatggg
cacaagggctggttcctattctgacgaaggctcacagaagaaatctgctg
gagctggacgtgataatgattggaagtgccctaattgcgacaacattaac
ttcgcctttcggacagtctgtaacatgagaaaatgcaacacaccaagacc
tgacacc

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