GrainGenes Sequence Report: NSFT03P2_Contig10201
Sequence
BE585732
Contig
NSFT03P2_Contig10201
External Databases
Data at GenBank Data at EMBL Data at DDBJ
TIGR Gene Index
TC279275
wEST map position
BE585732
NCBI UniGene
Ta.36181
DB Remark
Locus Source: Triticum aestivum (bread wheat) UniGene title 'Transcribed locus, moderately similar to NP_001050326.1 Os03g0404800 [Oryza sativa (japonica cultivar-group)]'
Keyword
EST
Species
Triticum aestivum
Cultivar
Sumai3
Chromosome
5AS 5DS
Clone Library
KSU wheat Fusarium graminearum infected spike cDNA library
Tissue
Spike
Developmental Stage
Adult plant
Data Source
genbank Release 135, Apr 15 2003 genbank Updated Nov 2006
Title
Est#1SP6_B12_b12_093 KSU wheat Fusarium graminearum infected spike cDNA library Triticum aestivum cDNA clone Est#1SP6_B12_b12_093, mRNA sequence.
Strain
lab_host E. coli JM109
Clone
Est#1SP6_B12_b12_093
Probe
MAG2919 Est#1SP6_B12_b12_093
Remark
DB_xref: taxon:4565 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: John Fellers; US Department of Agriculture, Agriculture Research Service, Plant; Science and Entomology Unit; Dept. of Plant Pathology, 4006 Throckmorton Hall, Kansas State; University, Manhattan, KS 66506, USA; Tel: 785-532-2367; Fax: 785-532-6167; Email: jpf@alfalfa.ksu.edu; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: SP6. Note: Vector: pGEM-T easy; Site_1: SacII; Site_2: SpeI; Plants were grown in the greenhouse. Spikes were sprayed with Fusarium graminearum (at what stage). Total RNA, and poly(A) RNA were prepared from infected spikes. cDNA was prepared using the SmartTM PCR cDNA synthesis kit from Clonetech. cDNA was cloned into the pGEM-T easy vector from Promega.
DNA
gcggggaggccttcggctaacattggctgctaaggcggctaaatacacaa
gacagagggaagcaagtacttaacggtagctagcagtgccactgcccact
agctagctcatccatggatatgtgcccggttggcggctgcgaccgccggg
aaccacgtcgtttgtgcgcggtgaccggtgggcggggattcatggcgagt
cacctggtgacggcgctgctcggctcgggagattggtctgtgcggatcac
cgaccttggcccccaggctgccctgtctcctgccgagagtgatggactcc
tgggcgctgccctccgcgatggccgcgccgcctacgtctctgtcgacgtc
tgcgaattagcccagcttacaaaagctttggaaggggtagatactgtttt
ccacactgctgcggcggatcataccaacaacaacttccaacttcattaca
aggttaatgtcgagggtacgaggaatgtcatcgaggcttgtaacacatgc
aaggttaaaacactcatatatactagttccagtggagttgtattcgatgg
agttcatggcctctttggcgtagacgaatctacaccgtatccagataagt
ttcccgacgcatacacagagacaaaggcagaagcagaaaagatggtgata
aagtccaacggaagaaatgagcttgtcact
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Sequence Report: NSFT03P2_Contig10201
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||