GrainGenes Sequence Report: NSFT03P2_Contig12383
Sequence
BE585722
Contig
Ta.622.1.S1_at Ta.622.3.S1_a_at NSFT03P2_Contig12383
External Databases
Data at GenBank Data at EMBL Data at DDBJ
TIGR Gene Index
TC310222
wEST map position
BE585722
NCBI UniGene
Ta.622
DB Remark
Locus Source: Triticum aestivum (bread wheat) UniGene title 'AML15'
Keyword
EST
Species
Triticum aestivum
Cultivar
Sumai3
Chromosome
4AL 7AS 7DS
Clone Library
KSU wheat Fusarium graminearum infected spike cDNA library
Tissue
Spike
Developmental Stage
Adult plant
Data Source
genbank Release 135, Apr 15 2003 genbank Updated Nov 2006
Title
Est#1SP6_A07_a7_049 KSU wheat Fusarium graminearum infected spike cDNA library Triticum aestivum cDNA clone Est#1SP6_A07_a7_049, mRNA sequence.
Strain
lab_host E. coli JM109
Clone
Est#1SP6_A07_a7_049
Probe
Est#1SP6_A07_a7_049
Remark
DB_xref: taxon:4565 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: John Fellers; US Department of Agriculture, Agriculture Research Service, Plant; Science and Entomology Unit; Dept. of Plant Pathology, 4006 Throckmorton Hall, Kansas State; University, Manhattan, KS 66506, USA; Tel: 785-532-2367; Fax: 785-532-6167; Email: jpf@alfalfa.ksu.edu; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: SP6. Note: Vector: pGEM-T easy; Site_1: SacII; Site_2: SpeI; Plants were grown in the greenhouse. Spikes were sprayed with Fusarium graminearum (at what stage). Total RNA, and poly(A) RNA were prepared from infected spikes. cDNA was prepared using the SmartTM PCR cDNA synthesis kit from Clonetech. cDNA was cloned into the pGEM-T easy vector from Promega.
DNA Homology
Ta.622.1.S1_at 1285 WHE2AFFY
DNA
gcggggagctcgagtcctgggactttaacagggcccgagtttctgtgggg
cagcccaaagccttatccggagcattcccaatcctcatcaatgtggcgcc
caccaccagttggtcatgcaatgtcatctagtagccgccctcagggtcag
ggcttcctgtatggtagtcgccaggcttcattgttcggatctttggatca
aaaccgtcatcatgttgggtctgccccttctggcgctccatttgagagcc
actttggatttttgcctgaatcgcctgagacgtcgtttatgaatcaagtt
aggtttgcaaatatggggaacattggcggtgctaaccgaaacgggggaag
cctcatgctgaacatggcttctcgtgcttccctgaatcctgtttccgctc
taagtggaagcctgacagataataactctactaacttcagaccagtaccg
tcgccaaggcttgggcaaccaccattctttgggaacaccacatatcaagg
acctggttattatggacttgacagctcctccattgagcgtggtcgtaacc
gccgagttgacagcagcgcgttccaggcagatagcaagaagcagtatcag
cttgatttggacaagatccgtaaaggagaggatactcggacaacattgat
gataaaaaacattccanacaagtacacatccaaan
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Sequence Report: NSFT03P2_Contig12383
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||