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GrainGenes Sequence Report: BE585671

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Sequence
BE585671
Contig
Ta.14021.1.S1_at
TaAffx.55762.1.S1_s_at
NSFT03P2_Contig14543
External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
TIGR Gene Index
TC289731
NCBI UniGene
Ta.14021
DB Remark
Locus Source: Triticum aestivum (bread wheat)
UniGene title 'Transcribed locus, strongly similar to NP_001054834.1 Os05g0187000 [Oryza sativa (japonica cultivar-group)]'
Keyword
EST
Species
Triticum aestivum
Cultivar
Sumai3
Clone Library
KSU wheat Fusarium graminearum infected spike cDNA library
Tissue
Spike
Developmental Stage
Adult plant
Data Source
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
EST2-3_A04_2F5t7_021 KSU wheat Fusarium graminearum infected spike cDNA library Triticum aestivum cDNA clone EST2-3_A04_2F5t7_021, mRNA sequence.
Strain
lab_host E. coli JM109
Clone
EST2-3_A04_2F5t7_021
Remark
DB_xref: taxon:4565
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: John Fellers; US Department of Agriculture, Agriculture Research Service, Plant; Science and Entomology Unit; Dept. of Plant Pathology, 4006 Throckmorton Hall, Kansas State; University, Manhattan, KS 66506, USA; Tel: 785-532-2367; Fax: 785-532-6167; Email: jpf@alfalfa.ksu.edu; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: T7.
Note: Vector: pGEM-T easy; Site_1: SacII; Site_2: SpeI; Plants were grown in the greenhouse. Spikes were sprayed with Fusarium graminearum (at what stage). Total RNA, and poly(A) RNA were prepared from infected spikes. cDNA was prepared using the SmartTM PCR cDNA synthesis kit from Clonetech. cDNA was cloned into the pGEM-T easy vector from Promega.
DNA
gcgggggaagcacaggaacaggaagcaagctgcccccccccccctcctca
tctccgactcactcacgatccgagatcaggatggccggatcgatggaact
ccctaccaagggcggcttcagcttcgacctgtgccgcaggaacgccatgc
tggagaagaatggcctcaagatgcccgggttcaggaagaccgggactacc
atcgtcggcctcgtgtttgcggatggagttgttcttggcgcagacacaag
ggcaaccgagggtcctatagttgctgataagaactgcgagaaaattcatt
ttatggcaccaaacatatattgctgtggtgctggaactgctgctgacaca
gaggctgtgacagatatggtcagctcccagctacaacttcaccgttatgc
tactagccgtgagtcaagagttgtaaccgctcttacactactgaagacac
acctttttaactaccaaggtcatgtcagtgctgctcttgttcttggcgga
gtagatgtcactggaccacatctacacactgtttatccgcatggttccac
cgacactcttccttttgccacgatgggttctggatcccttgctgcaatgt
cagtgtttgagtcgaagtacagagaaggcctgacaagggaggagggaatt
gcacttgtagccgaggcaatccgtgctgg

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