GrainGenes Sequence Report: BE585634

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Sequence

BE585634

External Databases

Data at GenBank

Data at EMBL

Data at DDBJ

DB Remark

Locus Source: Triticum aestivum (bread wheat)

Keyword

EST

Species

Triticum aestivum

Cultivar

Sumai3

Clone Library

KSU wheat Fusarium graminearum infected spike cDNA library

Tissue

Spike

Developmental Stage

Adult plant

Data Source

genbank	Release 135, Apr 15 2003
genbank	Updated Nov 2006

Title

EST#5T7_F03_f3_027 KSU wheat Fusarium graminearum infected spike cDNA library Triticum aestivum cDNA clone EST#5T7_F03_f3_027, mRNA sequence.

Strain

lab_host E. coli JM109

Clone

EST#5T7_F03_f3_027

Probe

MAG2913

Remark

DB_xref: taxon:4565

Feature: source: mol_type = 'mRNA';

Locus Comment: Contact: John Fellers; US Department of Agriculture, Agriculture Research Service, Plant; Science and Entomology Unit; Dept. of Plant Pathology, 4006 Throckmorton Hall, Kansas State; University, Manhattan, KS 66506, USA; Tel: 785-532-2367; Fax: 785-532-6167; Email: jpf@alfalfa.ksu.edu; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: T7.

Note: Vector: pGEM-T easy; Site_1: SacII; Site_2: SpeI; Plants were grown in the greenhouse. Spikes were sprayed with Fusarium graminearum (at what stage). Total RNA, and poly(A) RNA were prepared from infected spikes. cDNA was prepared using the SmartTM PCR cDNA synthesis kit from Clonetech. cDNA was cloned into the pGEM-T easy vector from Promega.

DNA

gcgggggtggaagtctactgacgcggagcagttgaacaaccaaggccttt
atgttctatcatctcactgcccctcgctgtctgatcttgctttaagcttc
tgctcatacatcgacgacactggtcttggttatctatctgactgcaaaaa
attgaggtccctcaggctgaaccgtgcaccagcagtcacttctactggga
tttttcgggtggcggttggttgcaggtatctgtcagttctccaccttgtt
gactgtaaggcagtagacaacagggagtggcttgagtactttgggaggta
tggatcgttgggtgaacttgttgtaaaggattgtgatggaatcagccagt
atgacctcttaaagtttggcgcaggatgggcaaatctccaaaagtttgag
ttcgagattaatggagattatttcatgttatttgctcatgatccctccat
cgtgtctggctacccatatagctatgacatctgctgtgatagcttgaagg
atcttcggttggctcatattataacaaatgaagaaattggacttcgtttt
ctcctggggaagtgcaaagcattagagacactttacctggagtatgttat
tggtctacatgagaatgagatgatttcactattccagaggtgcagcaacc
ttaaaactgtcgcacttcgactcangcctctgcgctgtgaagatttgagt
ataggacagcactgactg


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