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GrainGenes Sequence Report: BE585557

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Sequence
BE585557
External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
TIGR Gene Index
TC308611
wEST map position
BE585557
NCBI UniGene
Ta.8292
DB Remark
Locus Source: Triticum aestivum (bread wheat)
UniGene title 'Transcribed locus, weakly similar to NP_001049480.1 Os03g0234500 [Oryza sativa (japonica cultivar-group)]'
Keyword
EST
Species
Triticum aestivum
Cultivar
Sumai3
Chromosome
2DL
3AL
Clone Library
KSU wheat Fusarium graminearum infected spike cDNA library
Tissue
Spike
Developmental Stage
Adult plant
Data Source
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
EST-426samp_F06_1H8t7_047 KSU wheat Fusarium graminearum infected spike cDNA library Triticum aestivum cDNA clone EST-426samp_F06_1H8t7_047, mRNA sequence.
Strain
lab_host E. coli JM109
Clone
EST-426samp_F06_1H8t7_047
Probe
MAG2906
EST-426samp_F06_1H8t7_047
Remark
DB_xref: taxon:4565
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: John Fellers; US Department of Agriculture, Agriculture Research Service, Plant; Science and Entomology Unit; Dept. of Plant Pathology, 4006 Throckmorton Hall, Kansas State; University, Manhattan, KS 66506, USA; Tel: 785-532-2367; Fax: 785-532-6167; Email: jpf@alfalfa.ksu.edu; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: T7.
Note: Vector: pGEM-T easy; Site_1: SacII; Site_2: SpeI; Plants were grown in the greenhouse. Spikes were sprayed with Fusarium graminearum (at what stage). Total RNA, and poly(A) RNA were prepared from infected spikes. cDNA was prepared using the SmartTM PCR cDNA synthesis kit from Clonetech. cDNA was cloned into the pGEM-T easy vector from Promega.
DNA Homology
Ta.2746.1.S1_a_at76WHE2AFFY
[ Show all 13 ]
DNA
gcggggacatccatcttctacgatattccttcattcccttctagcagcta
gcttgccaatccatccctcagtggcaaattaagcagcttaattctagcct
tctccttctgaacgtgcagtggtgcacatggcggcttcctcttgcttccc
gttagctgctcgctgcagcctcttgctcacggcagcgctagtcctagcgc
taagccatggagcccacggttacggcgatgccggtgccgggttgagctcg
tccttctacgacgactcgtgccccggtgcgcgcgacattgttcggcgcgt
catccagaacgcccgtaccgtcgacgcgcgcatcccagccagcctcatcc
gccttcacttccatgattgctttgttcagggttgtgacggctcgcttttg
ctggacaacgacctcccagcgatcatgactgagaaggaggtccctgccaa
cgacaggtcagcacgcgggttcaaggtggtcgacagcatcaagcgtgcac
tggagaacgcatgccctggcatcgtgtcctgcgccgacatcctcgccctc
gcctctgagatctccgtcgagctggctggagggccacgctggagtgtgcc
gcttggccgccgtgatggcacgaccaccaacatcgagagcgccaacaatc
taccgagccccttcaactctctagagan

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