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GrainGenes Sequence Report: BE585492

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Sequence
BE585492
External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
TIGR Gene Index
TC362315
NCBI UniGene
Ta.35029
DB Remark
Locus Source: Triticum aestivum (bread wheat)
UniGene title 'Transcribed locus, weakly similar to NP_001067871.1 Os11g0469900 [Oryza sativa (japonica cultivar-group)]'
Keyword
EST
Species
Triticum aestivum
Cultivar
Sumai3
Clone Library
KSU wheat Fusarium graminearum infected spike cDNA library
Tissue
Spike
Developmental Stage
Adult plant
Data Source
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
EST#6PT7_B06_b6_045 KSU wheat Fusarium graminearum infected spike cDNA library Triticum aestivum cDNA clone EST#6PT7_B06_b6_045, mRNA sequence.
Strain
lab_host E. coli JM109
Clone
EST#6PT7_B06_b6_045
Probe
MAG2899
Remark
DB_xref: taxon:4565
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: John Fellers; US Department of Agriculture, Agriculture Research Service, Plant; Science and Entomology Unit; Dept. of Plant Pathology, 4006 Throckmorton Hall, Kansas State; University, Manhattan, KS 66506, USA; Tel: 785-532-2367; Fax: 785-532-6167; Email: jpf@alfalfa.ksu.edu; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: T7.
Note: Vector: pGEM-T easy; Site_1: SacII; Site_2: SpeI; Plants were grown in the greenhouse. Spikes were sprayed with Fusarium graminearum (at what stage). Total RNA, and poly(A) RNA were prepared from infected spikes. cDNA was prepared using the SmartTM PCR cDNA synthesis kit from Clonetech. cDNA was cloned into the pGEM-T easy vector from Promega.
DNA
gcggggtttcacaaatgaatgttagctatctcacactaagatacatgcga
tcataggtgagcttggtgatggccaagattaatgaggtggccacaaccga
tggtacggtggacatgagagagcttctcagcttattcgcgaatgacatgg
ggtgccgcatcgtatcgggaaagttcttcctaaaagatggacagagcaag
ttgttccgggacctcaccaacgatacctcacggctgttaggagggttcaa
cgtggaggagtacttcccaggattggcaagggtaggagtgtttaagaggg
cagtttgtgcccaagccgagagagtgaggaatagatgggctgatctgcta
gacaaggtgatcgacgatcatgtgagcaaggataagtcaacgtttgatca
cgaggatggtgattttgtagatattctgttgacttttcagcacgagtatg
atctcacaagagagcacatgaaagctatcctgatagatgtatttgccggt
gcaacagacacatcagctaacgccctcgaattcaccttggccgagctcat
gaggaagccacgtgtgatggggaagctacaagctgaggtgaggagtaacg
taccccagggacaagaaattgtcagtgaaatcaacgtgaacaatatggca
tacctaagagcagtcataaaggagtcgctttgactgtatcctatgcgcct
cttcttgctccacacct

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