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GrainGenes Sequence Report: BE517833

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Sequence
BE517833
Contig
Ta.1317.1.S1_at
NSFT03P2_Contig17744
Tracefile
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External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
TIGR Gene Index
TC283459
NCBI UniGene
Ta.48628
DB Remark
Locus Source: Triticum aestivum (bread wheat)
UniGene title 'Initiation factor (iso)4F p28 subunit'
Keyword
EST
Germplasm
Chinese Spring
Species
Triticum aestivum
Cultivar
Chinese Spring
Clone Library
Wheat vernalized crown cDNA library
Tissue
Crown tissue of seedling
Developmental Stage
Five-week old seedling
Data Source
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
WHE0803_C08_F15ZS Wheat vernalized crown cDNA library Triticum aestivum cDNA clone WHE0803_C08_F15, mRNA sequence.
Other Name
WHE0803_C08_F15ZS  [ wEST-mySQL (obsolete) ]
Strain
lab_host E. coli SOLR
DNA Library
TA016E1X
Clone
WHE0803_C08_F15
Probe
[Wcl619ct1438cn1980]
{SpCl-950}
Remark
DB_xref: taxon:4565
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: Stratagene SK primer.
Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Seeds were germinated and grown at 4 C for 5 weeks. The tissue, total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA Homology
Ta.1317.1.S1_at682WHE2AFFY
DNA
aaagcggcggcgcgacatggcagaggtcgaagctgcgctcccggtggcgg
cgacagagaccccggaggtcgcggccgagagcgacgccggtgcggccgag
gcgaaggggccgcacaagctgcagcggcagtggaccttctggtacgacat
ccagaccaagcccaagcccggcgccgcctgtggcacctcgctcaaaaagg
gctacaccttcgacaccgtcgaagagttctggtgcttgtatgatcagatt
ttccgtccgagtaagctggtaggaagtgctgattttcatttattcaaggc
tggggtagaaccaaagtgggaagatccagagtgtgcaaatgggggcaaat
ggactgtgatttc

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