Query (optional)   in Class  

GrainGenes Sequence Report: NSFT03P2_Contig5401

[Submit comment/correction]

Sequence
BE517815
Contig
Ta.23685.1.A1_a_at
Ta.23685.1.A1_x_at
NSFT03P2_Contig5401
Tracefile
[ Download ]
[ View ]
External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
TIGR Gene Index
TC324329
NCBI UniGene
Ta.23685
DB Remark
Locus Source: Triticum aestivum (bread wheat)
UniGene title 'Transcribed locus, moderately similar to NP_001058649.1 Os06g0729400 [Oryza sativa (japonica cultivar-group)]'
Keyword
EST
Germplasm
Chinese Spring
Species
Triticum aestivum
Cultivar
Chinese Spring
Clone Library
Wheat vernalized crown cDNA library
Tissue
Crown tissue of seedling
Developmental Stage
Five-week old seedling
Data Source
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
WHE0803_E02_J03ZS Wheat vernalized crown cDNA library Triticum aestivum cDNA clone WHE0803_E02_J03, mRNA sequence.
Other Name
WHE0803_E02_J03ZS  [ wEST-mySQL (obsolete) ]
Strain
lab_host E. coli SOLR
DNA Library
TA016E1X
Clone
WHE0803_E02_J03
Probe
CFE249
Remark
DB_xref: taxon:4565
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: Stratagene SK primer.
Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Seeds were germinated and grown at 4 C for 5 weeks. The tissue, total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA
catcctggcgccgacccagcagcggcttctcactcgtcgccgtctccttc
ctcctctgctgctgttgctcctcctcgccgcacatcttcagcctgctgct
gcttcctcttctgcttctgatccgctggtgaccaccacgacggcccacgg
cagcatgagggccagcagcaggtccctgctccagcagcagccgcctccca
gactagactgccccaaggtgtgcttgggccgctgcgccaacaactggaag
aacgagatgtgcaacgacaagtgcaacgtctgctgccagcgctgcaactg
cgtgccccccggcaccggccaggacacccgccacatctgcccctgctacg
accaaatgaccaacccgcacaacggcaagctcaagtgcccttgatctaga
gcacatcatcttcagatgtgtcttctgtcatgcatgttctactgttccca
ccctcagggagtaatggcctgtgatcttatcaaagcataa

GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.