GrainGenes Sequence Report: BE516546

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Sequence

BE516546

Contig

TaAffx.43576.1.A1_at

NSFT03P2_Contig10892

Tracefile

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External Databases

Data at GenBank

Data at EMBL

Data at DDBJ

DB Remark

Locus Source: Triticum aestivum (bread wheat)

Keyword

EST

Germplasm

BREVOR

Species

Triticum aestivum

Cultivar

Brevor (soft, white, winter, common wheat)

Clone Library

Wheat ABA-treated embryo cDNA library

Tissue

Seed embryo

Developmental Stage

Mature dormant seeds

Data Source

genbank	Release 135, Apr 15 2003
genbank	Updated Nov 2006

Title

WHE611_A02_B03ZA Wheat ABA-treated embryo cDNA library Triticum aestivum cDNA clone WHE611_A02_B03, mRNA sequence.

Other Name

WHE611_A02_B03ZA [ wEST-mySQL (obsolete) ]

Strain

lab_host E. coli DH12S

DNA Library

TA012XXX

Clone

WHE611_A02_B03

Probe

[BE516546.1]

{SpCl-294}

Remark

DB_xref: taxon:4565

Feature: source: mol_type = 'mRNA';

Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: Clontech Matchmaker 3' AD primer.

Note: Vector: pGAD10; Site_1: EcoRI; Site_2: XhoI; Embryos were cut from mature, dormant seeds and imbibed in 25 microM ABA (abscisic acid) in 5 mM Mes buffer, pH 5.7, for 12 hr at 22 C. The tissue, total RNA, and poly(A) RNA were prepared by Steven Verhey in M.K. Walker-Simmons's lab (USDA-ARS, Washington State Univ., Pullman, Washington 99164-6420. A cDNA library was made by Clontech using a combination of random and oligo dT primers. Library was plated and archived by Russell Johnson (Colby College, ME/Walker-Simmons' lab). Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).

DNA

cgaattcgcggccgcgtcgacctttattatccttcctcctcctcctgctg
ctgctgctgctggtcatggcggtcatggactcatggtggtgctggtgctg
gtgcttcctcacaagcagccgcccccgtagccggcgttcttgaacagctc
ctgcttgatggcgtccgcgctgatcatctccctctgcatctcggagcagg
aggcgtgcatggcgaagtcgttgaagcagctgacgacgcactgctggatg
tcgaggccgagggtgtcgagcgtgcccaccgtcgacagcagcagccccgg
cttggccgcgcagtagacctccacccgggtgtcgccctcctccttgcgct
ccacctcaactgggggtgttccgggcgagcatctcgttggggttgagctc
ccggaacacgctcagcagcggcgctgccgccgccgccgccggcccgggat
gctggtgcggacacgcctcctccatctcctcctgcagccgccggatcctc
tccagcagctccttcatgtagtcgatggtgtcccccaggatcgacgtcct
gtccatcttgctgatcttgggcaccacggagcggagcatggag


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