GrainGenes Sequence Report: Ta.1546.1.S1

GrainGenes Sequence Report: Ta.1546.1.S1_at

[Submit comment/correction]

Sequence

BE515393

Contig

Ta.1546.1.S1_at

NSFT03P2_Contig18634

Tracefile

[ Download ]

[ View ]

External Databases

TIGR Gene Index

NCBI UniGene

DB Remark

Locus Source: Triticum aestivum (bread wheat)

UniGene title 'Transcribed locus, strongly similar to NP_001056384.1 Os05g0573700 [Oryza sativa (japonica cultivar-group)]'

Keyword

EST

Germplasm

BREVOR

Species

Triticum aestivum

Cultivar

Brevor (soft, white, winter, common wheat)

Clone Library

Wheat ABA-treated embryo cDNA library

Tissue

Seed embryo

Developmental Stage

Mature dormant seeds

Data Source

genbank	Release 135, Apr 15 2003
genbank	Updated Nov 2006

Title

WHE0605_E11_I21ZA Wheat ABA-treated embryo cDNA library Triticum aestivum cDNA clone WHE0605_E11_I21, mRNA sequence.

Other Name

WHE0605_E11_I21ZA [ wEST-mySQL (obsolete) ]

Strain

lab_host E. coli DH12S

DNA Library

TA012XXX

Clone

WHE0605_E11_I21

Probe

[Wcl474ct1247cn1747]

Remark

DB_xref: taxon:4565

Feature: source: mol_type = 'mRNA';

Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: Clontech Matchmaker 3' AD primer.

Note: Vector: pGAD10; Site_1: EcoRI; Site_2: XhoI; Embryos were cut from mature, dormant seeds and imbibed in 25 microM ABA (abscisic acid) in 5 mM Mes buffer, pH 5.7, for 12 hr at 22 C. The tissue, total RNA, and poly(A) RNA were prepared by Steven Verhey in M.K. Walker-Simmons's lab (USDA-ARS, Washington State Univ., Pullman, Washington 99164-6420. A cDNA library was made by Clontech using a combination of random and oligo dT primers. Library was plated and archived by Russell Johnson (Colby College, ME/Walker-Simmons' lab). Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).

DNA

cgaattcgcggccgcgcgactggggatcctagtgcaaccgaccacccaaa
agcaacatcagtggcccttccatcaacatcctggtgaacagcaaagctag
cgttgatgccagcaccatttacttccttgccctgaacatacagtctccga
actgatggacccattcccttggggcatacagcaaccacgctgatgttctt
agggaaatcaaggccatgtgattgcagatgtccaaggagaaacccgtgag
ataaaccaagaatgctgtttggtttcatgtgagagaagatcttctcatag
ttgtctgcctgggcagaatctgagatcaacagcagcacaagatcactgcc
agagaccgtctcccatatgtctcccaaggttccgttctcctcggtgaatc
cagcaccacgtgcttcctcaaaagacttggaacctttcctgaggccaatc
ttgacgacgatgtcagacttggcttccaccaaggaatccctcaggttctg
ggcctgcgccggaccctgggagccccatccgatgaccccgatctgcttgt
gagcttgag


GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.