GrainGenes Sequence Report: Ta.10329.8.S1_x

GrainGenes Sequence Report: Ta.10329.8.S1_x_at

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Sequence

BE515379

Contig

Ta.10329.8.S1_x_at

NSFT03P2_Contig14324

Tracefile

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External Databases

TIGR Gene Index

NCBI UniGene

DB Remark

Locus Source: Triticum aestivum (bread wheat)

UniGene title 'Transcribed locus, strongly similar to NP_524352.1 histone H4 replacement, isoform C [Drosophila melanogaster]'

Keyword

EST

Germplasm

BREVOR

Species

Triticum aestivum

Cultivar

Brevor (soft, white, winter, common wheat)

Clone Library

Wheat ABA-treated embryo cDNA library

Tissue

Seed embryo

Developmental Stage

Mature dormant seeds

Data Source

genbank	Release 135, Apr 15 2003
genbank	Updated Nov 2006

Title

WHE0605_G02_M03ZA Wheat ABA-treated embryo cDNA library Triticum aestivum cDNA clone WHE0605_G02_M03, mRNA sequence.

Other Name

WHE0605_G02_M03ZA [ wEST-mySQL (obsolete) ]

Strain

lab_host E. coli DH12S

DNA Library

TA012XXX

Clone

WHE0605_G02_M03

Remark

DB_xref: taxon:4565

Feature: source: mol_type = 'mRNA';

Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: Clontech Matchmaker 3' AD primer.

Note: Vector: pGAD10; Site_1: EcoRI; Site_2: XhoI; Embryos were cut from mature, dormant seeds and imbibed in 25 microM ABA (abscisic acid) in 5 mM Mes buffer, pH 5.7, for 12 hr at 22 C. The tissue, total RNA, and poly(A) RNA were prepared by Steven Verhey in M.K. Walker-Simmons's lab (USDA-ARS, Washington State Univ., Pullman, Washington 99164-6420. A cDNA library was made by Clontech using a combination of random and oligo dT primers. Library was plated and archived by Russell Johnson (Colby College, ME/Walker-Simmons' lab). Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).

DNA

tcgcggccgcgtcgactttttttttttgttaactctggattcatatatct
cggcaaacacagtgtttgcccatcagaaattcagaacactgaagcaacca
ttgaacaaccagctactgctactacagatattactactaatcatagaagg
catggaacccagatcaactacagctttctcctctcctccactttcttgac
cgcgagccagggcttgtgcgccacggccggccagcaagcgccgcggctcc
tcagccgccgaatccgtacagggtgcgtccctggcgcttgagcgcgtaga
cgacgtccatggcggtgacggtcttccggcgggcgtgctcggtgtaggtg
acggcgtcgcggatgacgttctcgaggaagatcttgagcacgccgcgggt
ctcctcgtagatgagccccgagatgcgcttcacgccgcccctccgggcca
gcctccggatcgccggcttggtgatgccctggatgttgtcgcgcaggacc
ttgcggtggcgcttggcgcc


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