Query (optional)   in Class  

GrainGenes Sequence Report: BE500613

[Submit comment/correction]

Sequence
BE500613
Contig
Ta.8129.1.S1_at
Ta.8129.1.S1_s_at
Tracefile
[ Download ]
[ View ]
External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
TIGR Gene Index
TC301031
wEST map position
BE500613
NCBI UniGene
Ta.8129
DB Remark
Locus Source: Triticum aestivum (bread wheat)
UniGene title 'Transcribed locus, moderately similar to NP_001062826.1 Os09g0307300 [Oryza sativa (japonica cultivar-group)]'
Keyword
EST
Germplasm
Chinese Spring
Species
Triticum aestivum
Cultivar
Chinese Spring
Clone Library
Wheat pre-anthesis spike cDNA library
Tissue
Spike before anthesis
Developmental Stage
Adult plant
Data Source
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
WHE0987-0990_N03_N03ZS Wheat pre-anthesis spike cDNA library Triticum aestivum cDNA clone WHE0987-0990_N03_N03, mRNA sequence.
Other Name
WHE0987-0990_N03_N03ZS  [ wEST-mySQL (obsolete) ]
Strain
lab_host E. coli SOLR
DNA Library
TA019E1X
Clone
WHE0987-0990_N03_N03
Probe
[Wcl824ct1686cn2265]
{SpCl-676}
WHE0987-0990_N03_N03
Remark
DB_xref: taxon:4565
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: Stratagene SK primer.
Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown in the greenhouse. Whole spike with awns trimmed, white, green and yellow anther were collected and total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA Homology
Ta.8129.1.S1_at797WHE2AFFY
Ta.8129.1.S1_s_at797WHE2AFFY
TaAffx.85636.1.S1_at262WHE2AFFY
TaAffx.85636.1.S1_at230WHE2AFFY
Ta.29781.1.A1_at54WHE2AFFY
DNA
gagaaggcaaggaaggccacctcccctaagagctcatcaactagtccagg
cccgaaaagcccagcaaggagtggagggtcccctcctcataaaaagaacg
tcaccgaggcacgaccaaaaaatgagcagcaaaacttccgtaaaggcggt
caagactcggcaactcatgatgagagcaaacgacgttgaccaacttcaca
aacctcacccaagagatcgacaaaacacgagcagccgctgagctactgca
ggcttcacaccgaggagcgggccataaggcgagctggctacaattaccag
attgcaagcaagataaataccgaggaaattattcggagatttgaggacaa
gttatcacaggtgatggaggagcgcgaaatcaaaatgatgaggaaggaga
tggttccaaaggcccaacttatgccagcatttgacaagccattccatcca
caaaggtcgaggaggcccctcaccgtcccc

GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.