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GrainGenes Sequence Report: NSFT03P2_Contig17332

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Sequence
BE499835
Contig
Ta.3706.1.S1_at
NSFT03P2_Contig17332
Tracefile
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External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
TIGR Gene Index
TC283592
wEST map position
BE499835
NCBI UniGene
Ta.23445
DB Remark
Locus Source: Triticum aestivum (bread wheat)
UniGene title 'Transcribed locus, strongly similar to NP_001047033.1 Os02g0533900 [Oryza sativa (japonica cultivar-group)]'
Keyword
EST
Germplasm
Chinese Spring
Species
Triticum aestivum
Cultivar
Chinese Spring
Chromosome
5AS
5BS
5DS
Clone Library
Wheat pre-anthesis spike cDNA library
Tissue
Spike before anthesis
Developmental Stage
Adult plant
Data Source
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
WHE0979_F10_K19ZS Wheat pre-anthesis spike cDNA library Triticum aestivum cDNA clone WHE0979_F10_K19, mRNA sequence.
Other Name
WHE0979_F10_K19ZS  [ wEST-mySQL (obsolete) ]
Strain
lab_host E. coli SOLR
DNA Library
TA019E1X
Clone
WHE0979_F10_K19
Probe
MAG697
WHE0979_F10_K19
BE406584
BE499835
Remark
DB_xref: taxon:4565
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: Stratagene SK primer.
Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown in the greenhouse. Whole spike with awns trimmed, white, green and yellow anther were collected and total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA Homology
Ta.3706.1.S1_at900WHE2AFFY
Ta.3706.3.S1_x_at262WHE2AFFY
Ta.3706.3.S1_at262WHE2AFFY
DNA
cgcgaacattgtcctcattcaggagctatttgaggggcactatttctgtc
aggctcaaaggatggatttctttgggcgtgccaagccttataaagagaac
ccgactataatgagaatgcaaaagcttgcaaaggagttggatgttgtaat
acctgtaagtttctttgaagaagcgggcaatgctcattacaattccgtgg
ccattattgatgctgatggcaccgatcttggcctataccgcaaatcacac
attccagatggaccaggttatcaagagaaattttatttcaacccgggtga
cactggattcaaggctttcaaaaccaagtatgcaacaattggtgttggaa
tttgttgggaccagtggtttccagagaccgcaagggctatggtgctacag
ggggcggagatattgttctatcccactgctattggttctgaaccccagga
tatgaacctggattcccgcgaacactggaagcgtg

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