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GrainGenes Sequence Report: BE499622

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Sequence	BE499622
Contig	Ta.2319.2.S1_a_at Ta.2319.3.S1_a_at NSFT03P2_Contig18418
Tracefile	[ Download ] [ View ]
External Databases	Data at GenBank Data at EMBL Data at DDBJ
TIGR Gene Index	TC300541
wEST map position	BE499622
NCBI UniGene	Ta.1487
DB Remark	Locus Source: Triticum aestivum (bread wheat) UniGene title 'Transcribed locus, strongly similar to NP_001105534.1 meg5 protein [Zea mays]'
Keyword	EST
Germplasm	Chinese Spring
Species	Triticum aestivum
Cultivar	Chinese Spring
Chromosome	5BS 7AL 7BL 7DL
Clone Library	Wheat pre-anthesis spike cDNA library
Tissue	Spike before anthesis
Developmental Stage	Adult plant
Data Source	genbank Release 135, Apr 15 2003 genbank Updated Nov 2006
Title	WHE0962_D01_H02ZS Wheat pre-anthesis spike cDNA library Triticum aestivum cDNA clone WHE0962_D01_H02, mRNA sequence.
Other Name	WHE0962_D01_H02ZS  [ wEST-mySQL (obsolete) ]
Strain	lab_host E. coli SOLR
DNA Library	TA019E1X
Clone	WHE0962_D01_H02
Probe	[Wcl659ct1481cn2030] CFE221 WHE0962_D01_H02
Remark	DB_xref: taxon:4565 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: Stratagene SK primer. Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown in the greenhouse. Whole spike with awns trimmed, white, green and yellow anther were collected and total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA Homology	TaAffx.25029.1.S1_x_at 54 WHE2AFFY TaAffx.25029.1.S1_at 54 WHE2AFFY
DNA	ccgccgtctgacctgcctacgagggcgaacgatggccgcggagaactact ggaggtacgccgacgcgcggcagcagcaggccatggtggcggctgcggcg gccgcggcggggatggcccctgcagctaccgttgcgcacgccgcgacggc cgcagggggcatgcagtagcaccagcaccagcaccagcaccagcaggcgg cgatggcgcaacagcagcaacagcagcaacagcatgcagcacgcggccgc gccgtcgcttaagcgcgcgcgcccagactatggcgatggtcctggaggac aagagatggctggatattatccgcgggaaaatgcatgttaccactcactt agagacatgaagcaattggagcatcttacgaccgctacttacgcaatggg atgccttctgtggcagccaatgaaccaagtatggcagttggtggtgccat gggtggagctgcaatggttggtggtggaatg

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