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GrainGenes Sequence Report: BE499553

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Sequence
BE499553
Contig
Ta.9161.1.S1_at
NSFT03P2_Contig6617
Tracefile
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External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
TIGR Gene Index
TC343497
wEST map position
BE499553
NCBI UniGene
Ta.9161
DB Remark
Locus Source: Triticum aestivum (bread wheat)
UniGene title 'Transcribed locus'
Keyword
EST
Germplasm
Chinese Spring
Species
Triticum aestivum
Cultivar
Chinese Spring
Chromosome
2BL
5AL
6AL
6DL
Clone Library
Wheat pre-anthesis spike cDNA library
Tissue
Spike before anthesis
Developmental Stage
Adult plant
Data Source
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
WHE0961_B03_D05ZS Wheat pre-anthesis spike cDNA library Triticum aestivum cDNA clone WHE0961_B03_D05, mRNA sequence.
Other Name
WHE0961_B03_D05ZS  [ wEST-mySQL (obsolete) ]
Strain
lab_host E. coli SOLR
DNA Library
TA019E1X
Clone
WHE0961_B03_D05
Probe
WHE0961_B03_D05
Remark
DB_xref: taxon:4565
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: Stratagene SK primer.
Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown in the greenhouse. Whole spike with awns trimmed, white, green and yellow anther were collected and total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA Homology
Ta.9161.1.S1_at1241WHE2AFFY
Ta.9161.1.S1_at833WHE2AFFY
Ta.11963.1.A1_at204WHE2AFFY
Ta.11963.1.A1_at72WHE2AFFY
DNA
gccgcccctgcatcttgccaccacgcatcgagcgatgcagcactcagggt
cgtttcgccaccaccacctccggccgttcagcgatgcagcagcaagatgt
tcaacgcctcagcccccctgcgaatcccactgttgcagcaaccggacgag
gcccatatcttttttctttgataagataatttttattgcatactgtctgt
tattttgctctgtcattttgctgatgcctaaaaagaagttagatgatggg
ctctgcggtctgcaagctgcctccaacctttggcaatatttgtaaaaatg
attgtgcagattttttgttaccccattttagttactgattgaagaaaatt
ttgttcattaggtgaaagaacttgatttcttttgttcctttgtggaggaa
gaaggtcaccacagaggtagggagaaggtcaccataaaggcagggagaag
gtcacataaggtgcagaaagaatgtcagagttcagcaacggggtgtgatc
tttgtgttgtttttggggtgcaaatcatgggtatgcgatctcacatcctg
tgtactattgagatggcctcccttagcctcaaaccatattgcctacaaaa
atgatagtcgcaatgctaccgtcatgtgtgtaaaga

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