GrainGenes Sequence Report: BE499458
Sequence
BE499458
Contig
TaAffx.122554.1.S1_at
Tracefile
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External Databases
Data at GenBank Data at EMBL Data at DDBJ
TIGR Gene Index
TC339594
wEST map position
BE499458
NCBI UniGene
Ta.46189
DB Remark
Locus Source: Triticum aestivum (bread wheat) UniGene title 'Transcribed locus, weakly similar to NP_001054636.1 Os05g0146100 [Oryza sativa (japonica cultivar-group)]'
Keyword
EST
Germplasm
Chinese Spring
Species
Triticum aestivum
Cultivar
Chinese Spring
Chromosome
5AS 5BS
Clone Library
Wheat pre-anthesis spike cDNA library
Tissue
Spike before anthesis
Developmental Stage
Adult plant
Data Source
genbank Release 135, Apr 15 2003 genbank Updated Nov 2006
Title
WHE0974_C04_F08ZS Wheat pre-anthesis spike cDNA library Triticum aestivum cDNA clone WHE0974_C04_F08, mRNA sequence.
Other Name
WHE0974_C04_F08ZS [ wEST-mySQL (obsolete) ]
Strain
lab_host E. coli SOLR
DNA Library
TA019E1X
Clone
WHE0974_C04_F08
Probe
WHE0974_C04_F08
Remark
DB_xref: taxon:4565 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: Stratagene SK primer. Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown in the greenhouse. Whole spike with awns trimmed, white, green and yellow anther were collected and total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA Homology
TaAffx.122554.1.S1_at 611 WHE2AFFY TaAffx.122554.1.S1_at 246 WHE2AFFY TaAffx.122554.1.S1_at 309 WHE2AFFY TaAffx.122554.1.S1_at 178 WHE2AFFY
DNA
cccaaaccgtattccggcctctccggtgcagaggcagcggcggcggaggt
gagagagatgtcgagggccgtcaggatccgcggcgacgagctggagacga
cggagcgggaagcgaagaggcgaagcgtggcagaggagggggaggggaca
gctctccactccacaagccctagatctccgggcgagctagctgttcggac
ttcccatccagagatacacttggcgatcttcgagcccaacaaagcccaat
cggaagcaaaacaagatcgtgtgtacgatgtggtcgagtccgtggcggag
gaatcctccgatccacccagccccccgatctaccaaccctacattcccga
cgagctagttgatgatctggacgtactcgctgacttcgaggacgccaagg
ccaagtacgaagcagaacgggatcgtcgagctaacctcttcactatggag
catcactgctataaagcagcctcctccctgttcaagacccatcgcttgct
tcctattcttgaacaagcaagggatgcgacacttcttgctgctaaatctg
tgattttgctctcatcatttgtcgagagt
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Sequence Report: BE499458
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