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GrainGenes Sequence Report: NSFT03P2_Contig10463

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Sequence
BE499251
Contig
Ta.28322.1.S1_s_at
Ta.9500.1.S1_at
NSFT03P2_Contig10463
Tracefile
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External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
TIGR Gene Index
TC289498
wEST map position
BE499251
NCBI UniGene
Ta.49756
DB Remark
Locus Source: Triticum aestivum (bread wheat)
UniGene title 'Clone wre1n.pk0035.f6:fis, full insert mRNA sequence'
Keyword
EST
Germplasm
Chinese Spring
Species
Triticum aestivum
Cultivar
Chinese Spring
Chromosome
2AL
2BL
2DL
Clone Library
Wheat pre-anthesis spike cDNA library
Tissue
Spike before anthesis
Developmental Stage
Adult plant
Data Source
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
WHE0972_E08_I16ZS Wheat pre-anthesis spike cDNA library Triticum aestivum cDNA clone WHE0972_E08_I16, mRNA sequence.
Other Name
WHE0972_E08_I16ZS  [ wEST-mySQL (obsolete) ]
Strain
lab_host E. coli SOLR
DNA Library
TA019E1X
Clone
WHE0972_E08_I16
Probe
WHE0972_E08_I16
Remark
DB_xref: taxon:4565
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: Stratagene SK primer.
Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown in the greenhouse. Whole spike with awns trimmed, white, green and yellow anther were collected and total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA Homology
Ta.9500.1.S1_at936WHE2AFFY
[ Show all 6 ]
DNA
caagacttggccggccggccggaggagatcgagaaggaggaggagacatg
gtgcgcgggaagctggaggtgctgctcgtctccgccaagggcctcgacga
ctccgatttcttcaatagcatggacccgtacgtgatcctcacctgccgca
gccacgagcagaagagcaccgtcgcatcaggagcagggagcgagcctgag
tggaatgagaccttcttcttcgccatctccggcgacgctccggagctcag
ggtcaagatcatggacagcgacgccctctcggccgacgacctcgtcggag
aagcatgcatcccgctggaggcggtgcttcaggaaggcagcctgccgccg
gccgtgcaccgggtcgtcaaggacgaggagtaccgcggagagatcaagat
cgcgctcaccttcaccccggcagaggaaaacgaggaggaggagagctacg
gcggctggaatcagtccacctg

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