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GrainGenes Sequence Report: NSFT03P2_Contig17866

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Sequence	BE498566
Contig	Ta.181.1.S1_at NSFT03P2_Contig17866
Tracefile	[ Download ] [ View ]
External Databases	Data at GenBank Data at EMBL Data at DDBJ
TIGR Gene Index	TC278472
wEST map position	BE498566
NCBI UniGene	Ta.181
DB Remark	Locus Source: Triticum aestivum (bread wheat) UniGene title 'Casein kinase II alpha'
Keyword	EST
Germplasm	Chinese Spring
Species	Triticum aestivum
Cultivar	Chinese Spring
Chromosome	2AL
Clone Library	Wheat pre-anthesis spike cDNA library
Tissue	Spike before anthesis
Developmental Stage	Adult plant
Data Source	genbank Release 135, Apr 15 2003 genbank Updated Nov 2006
Title	WHE0971_D01_G01ZS Wheat pre-anthesis spike cDNA library Triticum aestivum cDNA clone WHE0971_D01_G01, mRNA sequence.
Other Name	WHE0971_D01_G01ZS  [ wEST-mySQL (obsolete) ]
Strain	lab_host E. coli SOLR
DNA Library	TA019E1X
Clone	WHE0971_D01_G01
Probe	WHE0971_D01_G01
Remark	DB_xref: taxon:4565 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: Stratagene SK primer. Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown in the greenhouse. Whole spike with awns trimmed, white, green and yellow anther were collected and total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA Homology	Ta.181.1.S1_at 1168 WHE2AFFY [ Show all 8 ]
DNA	tgtgaagctgcttgatattgtcagggatcagcattcaaaaacaccaagct tgatctttgaatacatcaataacacagatttcaaagtgctatatccgaca ttgacagattatgacattcgctactatatctatgagttactgaaggcttt ggattactgccattcacaaggcattatgcaccgagatgtgaagcctcaca atgttatgatagatcatgagcttcgaaaactccggttaatagactggggc cttgctgaattctaccatcctggaaaggagtataacgttcgtgttgcatc aaggtacttcaagggacctgagcttcttgttgacttgcaagattacgact actctttggacatgtggagtcttggctgcatgtttgctggaatgatattc cgcaaggaaccatttttctatggccatgacaaccatgaccaacttgttaa aattgcaaaggtacttggaacagatgggctaaatgcttatttgaacaagt acagaattgagcttgaccctcagcttgaagcccttgttggaaggcacagc agaaaaccctggtcaaagtttatcaatgcagacaaccagcatctagtatc ccct

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